Metabolic profiling of serum using Ultra Performance Liquid Chromatography and the LTQ-Orbitrap mass spectrometry system

Warwick B Dunn; David Broadhurst; Marie Brown; Philip N Baker; Christopher W G Redman; Louise C Kenny; Douglas B Kell

doi:10.1016/j.jchromb.2008.03.021

Metabolic profiling of serum using Ultra Performance Liquid Chromatography and the LTQ-Orbitrap mass spectrometry system

Warwick B Dunn, David Broadhurst, Marie Brown, Philip N Baker, Christopher W G Redman, Louise C Kenny, Douglas B Kell

Biosciences

Research output: Contribution to journal › Article › peer-review

158 Citations (Scopus)

Abstract

Advances in analytical instrumentation can provide significant advantages to the volume and quality of biological knowledge acquired in metabolomic investigations. The interfacing of sub-2 microm liquid chromatography (UPLC ACQUITY) and LTQ-Orbitrap mass spectrometry systems provides many theoretical advantages. The applicability of the interfaced systems was investigated using a simple 11-component metabolite mix and a complex mammalian biofluid, serum. Metabolites were detected in the metabolite mix with signals that were linear with their concentration over 2.5-3.5 orders of magnitude, with correlation coefficients greater than 0.993 and limits of detection less than 1 micromol L(-1). Reproducibility of retention time (RSD

Original language	English
Pages (from-to)	288-98
Number of pages	11
Journal	Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
Volume	871
Issue number	2
DOIs	https://doi.org/10.1016/j.jchromb.2008.03.021
Publication status	Published - 15 Aug 2008

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10.1016/j.jchromb.2008.03.021

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Dunn, W. B., Broadhurst, D., Brown, M., Baker, P. N., Redman, C. W. G., Kenny, L. C., & Kell, D. B. (2008). Metabolic profiling of serum using Ultra Performance Liquid Chromatography and the LTQ-Orbitrap mass spectrometry system. Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, 871(2), 288-98. https://doi.org/10.1016/j.jchromb.2008.03.021

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title = "Metabolic profiling of serum using Ultra Performance Liquid Chromatography and the LTQ-Orbitrap mass spectrometry system",

abstract = "Advances in analytical instrumentation can provide significant advantages to the volume and quality of biological knowledge acquired in metabolomic investigations. The interfacing of sub-2 microm liquid chromatography (UPLC ACQUITY) and LTQ-Orbitrap mass spectrometry systems provides many theoretical advantages. The applicability of the interfaced systems was investigated using a simple 11-component metabolite mix and a complex mammalian biofluid, serum. Metabolites were detected in the metabolite mix with signals that were linear with their concentration over 2.5-3.5 orders of magnitude, with correlation coefficients greater than 0.993 and limits of detection less than 1 micromol L(-1). Reproducibility of retention time (RSD",

author = "Dunn, {Warwick B} and David Broadhurst and Marie Brown and Baker, {Philip N} and Redman, {Christopher W G} and Kenny, {Louise C} and Kell, {Douglas B}",

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doi = "10.1016/j.jchromb.2008.03.021",

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Dunn, WB, Broadhurst, D, Brown, M, Baker, PN, Redman, CWG, Kenny, LC & Kell, DB 2008, 'Metabolic profiling of serum using Ultra Performance Liquid Chromatography and the LTQ-Orbitrap mass spectrometry system', Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, vol. 871, no. 2, pp. 288-98. https://doi.org/10.1016/j.jchromb.2008.03.021

Metabolic profiling of serum using Ultra Performance Liquid Chromatography and the LTQ-Orbitrap mass spectrometry system. / Dunn, Warwick B; Broadhurst, David; Brown, Marie et al.
In: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, Vol. 871, No. 2, 15.08.2008, p. 288-98.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Metabolic profiling of serum using Ultra Performance Liquid Chromatography and the LTQ-Orbitrap mass spectrometry system

AU - Dunn, Warwick B

AU - Broadhurst, David

AU - Brown, Marie

AU - Baker, Philip N

AU - Redman, Christopher W G

AU - Kenny, Louise C

AU - Kell, Douglas B

PY - 2008/8/15

Y1 - 2008/8/15

N2 - Advances in analytical instrumentation can provide significant advantages to the volume and quality of biological knowledge acquired in metabolomic investigations. The interfacing of sub-2 microm liquid chromatography (UPLC ACQUITY) and LTQ-Orbitrap mass spectrometry systems provides many theoretical advantages. The applicability of the interfaced systems was investigated using a simple 11-component metabolite mix and a complex mammalian biofluid, serum. Metabolites were detected in the metabolite mix with signals that were linear with their concentration over 2.5-3.5 orders of magnitude, with correlation coefficients greater than 0.993 and limits of detection less than 1 micromol L(-1). Reproducibility of retention time (RSD

AB - Advances in analytical instrumentation can provide significant advantages to the volume and quality of biological knowledge acquired in metabolomic investigations. The interfacing of sub-2 microm liquid chromatography (UPLC ACQUITY) and LTQ-Orbitrap mass spectrometry systems provides many theoretical advantages. The applicability of the interfaced systems was investigated using a simple 11-component metabolite mix and a complex mammalian biofluid, serum. Metabolites were detected in the metabolite mix with signals that were linear with their concentration over 2.5-3.5 orders of magnitude, with correlation coefficients greater than 0.993 and limits of detection less than 1 micromol L(-1). Reproducibility of retention time (RSD

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SN - 1570-0232

VL - 871

SP - 288

EP - 298

JO - Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences

JF - Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences

IS - 2

ER -

Metabolic profiling of serum using Ultra Performance Liquid Chromatography and the LTQ-Orbitrap mass spectrometry system

Abstract

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