Membrane trafficking of aquaporin 1 is mediated by protein kinase C via microtubules and regulated by tonicity.
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Membrane trafficking of aquaporin 1 is mediated by protein kinase C via microtubules and regulated by tonicity. / Conner, Matthew; Conner, Alex; Brown, JE; Bill, RM.
In: Biochemistry, Vol. 49, No. 5, 09.02.2010, p. 821-3.Research output: Contribution to journal › Article
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TY - JOUR
T1 - Membrane trafficking of aquaporin 1 is mediated by protein kinase C via microtubules and regulated by tonicity.
AU - Conner, Matthew
AU - Conner, Alex
AU - Brown, JE
AU - Bill, RM
PY - 2010/2/9
Y1 - 2010/2/9
N2 - It is well-known that the rapid flow of water into and out of cells is controlled by membrane proteins called aquaporins (AQPs). However, the mechanisms that allow cells to quickly respond to a changing osmotic environment are less well established. Using GFP-AQP fusion proteins expressed in HEK293 cells, we demonstrate the reversible manipulation of cellular trafficking of AQP1. AQP1 trafficking was mediated by the tonicity of the cell environment in a specific PKC- and microtubule-dependent manner. This suggests that the increased level of water transport following osmotic change may be due a phosphorylation-dependent increase in the level of AQP1 trafficking resulting in membrane localization.
AB - It is well-known that the rapid flow of water into and out of cells is controlled by membrane proteins called aquaporins (AQPs). However, the mechanisms that allow cells to quickly respond to a changing osmotic environment are less well established. Using GFP-AQP fusion proteins expressed in HEK293 cells, we demonstrate the reversible manipulation of cellular trafficking of AQP1. AQP1 trafficking was mediated by the tonicity of the cell environment in a specific PKC- and microtubule-dependent manner. This suggests that the increased level of water transport following osmotic change may be due a phosphorylation-dependent increase in the level of AQP1 trafficking resulting in membrane localization.
U2 - 10.1021/bi902068b
DO - 10.1021/bi902068b
M3 - Article
C2 - 20063900
VL - 49
SP - 821
EP - 823
JO - Biochemistry
JF - Biochemistry
SN - 0006-2960
IS - 5
ER -