Membrane blebbing during apoptosis results from caspase-mediated activation of ROCK I
Research output: Contribution to journal › Article › peer-review
Colleges, School and Institutes
The execution phase of apoptosis is characterized by marked changes in cell morphology that include contraction and membrane blebbing. The actin-myosin system has been proposed to be the source of contractile force that drives bleb formation, although the biochemical pathway that promotes actin-myosin contractility during apoptosis has not been identified. Here we show that the Rho effector protein ROCK I, which contributes to phosphorylation of myosin light-chains, myosin ATPase activity and coupling of actin-myosin filaments to the plasma membrane, is cleaved during apoptosis to generate a truncated active form. The activity of ROCK proteins is both necessary and sufficient for formation of membrane blebs and for re-localization of fragmented DNA into blebs and apoptotic bodies.
|Number of pages||7|
|Journal||Nature Cell Biology|
|Publication status||Published - Apr 2001|
- 3T3 Cells, Animals, Apoptosis, Caspase Inhibitors, Caspases, Cell Membrane, DNA, DNA Fragmentation, Enzyme Activation, Humans, Intracellular Signaling Peptides and Proteins, Mice, Protein-Serine-Threonine Kinases, Recombinant Fusion Proteins, Tumor Cells, Cultured, rho-Associated Kinases