Maps of in vivo oxygen pressure with submillimetre resolution and nanomolar sensitivity enabled by Cherenkov-excited luminescence scanned imaging

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Maps of in vivo oxygen pressure with submillimetre resolution and nanomolar sensitivity enabled by Cherenkov-excited luminescence scanned imaging. / Pogue, Brian W.; Feng, Jinchao; LaRochelle, Ethan P. ; Bruza, Petr; Lin, Huiyun; Zhang, Rongxiao; Shell, Jennifer R.; Dehghani, Hamid; Davis, Scott C.; Vinogradov, Sergei A.; Gladstone, David J.

In: Nature Biomedical Engineering, Vol. 2, 13.04.2018, p. 254-264.

Research output: Contribution to journalArticlepeer-review

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APA

Pogue, B. W., Feng, J., LaRochelle, E. P., Bruza, P., Lin, H., Zhang, R., Shell, J. R., Dehghani, H., Davis, S. C., Vinogradov, S. A., & Gladstone, D. J. (2018). Maps of in vivo oxygen pressure with submillimetre resolution and nanomolar sensitivity enabled by Cherenkov-excited luminescence scanned imaging. Nature Biomedical Engineering, 2, 254-264. https://doi.org/10.1038/s41551-018-0220-3

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Author

Pogue, Brian W. ; Feng, Jinchao ; LaRochelle, Ethan P. ; Bruza, Petr ; Lin, Huiyun ; Zhang, Rongxiao ; Shell, Jennifer R. ; Dehghani, Hamid ; Davis, Scott C. ; Vinogradov, Sergei A. ; Gladstone, David J. / Maps of in vivo oxygen pressure with submillimetre resolution and nanomolar sensitivity enabled by Cherenkov-excited luminescence scanned imaging. In: Nature Biomedical Engineering. 2018 ; Vol. 2. pp. 254-264.

Bibtex

@article{337c79c38d2a4c849bd90724a2d34721,
title = "Maps of in vivo oxygen pressure with submillimetre resolution and nanomolar sensitivity enabled by Cherenkov-excited luminescence scanned imaging",
abstract = "Low signal to noise ratios and limited imaging depths restrict the ability of optical-imaging modalities to detect and accurately quantify molecular emissions from tissue. Here, by using a scanning external X-ray beam from a clinical linear accelerator to induce Cherenkov excitation of luminescence in tissue, we demonstrate in vivo mapping of the oxygenation of tumours at depths of several millimetres, with submillimetre resolution and nanomolar sensitivity. This was achieved by scanning thin sheets of the X-ray beam orthogonally to the emission-detection plane, and detecting the signal via a time-gated CCD camera synchronized to the radiation pulse. We also show with experiments using phantoms and with simulations that the performance of Cherenkov-excited luminescence scanned imaging (CELSI) is limited by beam size, scan geometry, probe concentration, radiation dose and tissue depth. CELSI might provide the highest sensitivity and resolution in the optical imaging of molecular tracers in vivo.",
author = "Pogue, {Brian W.} and Jinchao Feng and LaRochelle, {Ethan P.} and Petr Bruza and Huiyun Lin and Rongxiao Zhang and Shell, {Jennifer R.} and Hamid Dehghani and Davis, {Scott C.} and Vinogradov, {Sergei A.} and Gladstone, {David J.}",
year = "2018",
month = apr,
day = "13",
doi = "10.1038/s41551-018-0220-3",
language = "English",
volume = "2",
pages = "254--264",
journal = "Nature Biomedical Engineering",
issn = "2157-846X",
publisher = "Nature Publishing Group",

}

RIS

TY - JOUR

T1 - Maps of in vivo oxygen pressure with submillimetre resolution and nanomolar sensitivity enabled by Cherenkov-excited luminescence scanned imaging

AU - Pogue, Brian W.

AU - Feng, Jinchao

AU - LaRochelle, Ethan P.

AU - Bruza, Petr

AU - Lin, Huiyun

AU - Zhang, Rongxiao

AU - Shell, Jennifer R.

AU - Dehghani, Hamid

AU - Davis, Scott C.

AU - Vinogradov, Sergei A.

AU - Gladstone, David J.

PY - 2018/4/13

Y1 - 2018/4/13

N2 - Low signal to noise ratios and limited imaging depths restrict the ability of optical-imaging modalities to detect and accurately quantify molecular emissions from tissue. Here, by using a scanning external X-ray beam from a clinical linear accelerator to induce Cherenkov excitation of luminescence in tissue, we demonstrate in vivo mapping of the oxygenation of tumours at depths of several millimetres, with submillimetre resolution and nanomolar sensitivity. This was achieved by scanning thin sheets of the X-ray beam orthogonally to the emission-detection plane, and detecting the signal via a time-gated CCD camera synchronized to the radiation pulse. We also show with experiments using phantoms and with simulations that the performance of Cherenkov-excited luminescence scanned imaging (CELSI) is limited by beam size, scan geometry, probe concentration, radiation dose and tissue depth. CELSI might provide the highest sensitivity and resolution in the optical imaging of molecular tracers in vivo.

AB - Low signal to noise ratios and limited imaging depths restrict the ability of optical-imaging modalities to detect and accurately quantify molecular emissions from tissue. Here, by using a scanning external X-ray beam from a clinical linear accelerator to induce Cherenkov excitation of luminescence in tissue, we demonstrate in vivo mapping of the oxygenation of tumours at depths of several millimetres, with submillimetre resolution and nanomolar sensitivity. This was achieved by scanning thin sheets of the X-ray beam orthogonally to the emission-detection plane, and detecting the signal via a time-gated CCD camera synchronized to the radiation pulse. We also show with experiments using phantoms and with simulations that the performance of Cherenkov-excited luminescence scanned imaging (CELSI) is limited by beam size, scan geometry, probe concentration, radiation dose and tissue depth. CELSI might provide the highest sensitivity and resolution in the optical imaging of molecular tracers in vivo.

U2 - 10.1038/s41551-018-0220-3

DO - 10.1038/s41551-018-0220-3

M3 - Article

VL - 2

SP - 254

EP - 264

JO - Nature Biomedical Engineering

JF - Nature Biomedical Engineering

SN - 2157-846X

ER -