Manufacturing of agarose-based chromatographic adsorbents with controlled pore and particle sizes

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Authors

Colleges, School and Institutes

External organisations

  • Department of Chemical Engineering

Abstract

The effect of ionic strength and quenching temperature on the mechanical properties and structure of agarose-based chromatographic adsorbents in micro-beads for bioseparation was investigated. The pore size/size distribution of the beads was measured by analysis of their AFM images. Their mechanical properties were determined by a micromanipulation technique based on compression of single micro-particles. It was found that the mean pore size and stiffness of agarose beads increase with ionic strength and slow cooling. These two parameters do not affect the particle size/size distribution.

Details

Original languageEnglish
Title of host publicationAnnual Technical Conference - ANTEC, Conference Proceedings
Publication statusPublished - 2012
Event69th Annual Technical Conference of the Society of Plastics Engineers 2011, ANTEC 2011 - Boston, MA, United States
Duration: 1 May 20115 May 2011

Conference

Conference69th Annual Technical Conference of the Society of Plastics Engineers 2011, ANTEC 2011
CountryUnited States
CityBoston, MA
Period1/05/115/05/11