Manufacturing of agarose-based chromatographic adsorbents - Effect of ionic strength and cooling conditions on particle structure and mechanical strength.

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@article{8508515906924520be4a07940024bc1c,
title = "Manufacturing of agarose-based chromatographic adsorbents - Effect of ionic strength and cooling conditions on particle structure and mechanical strength.",
abstract = "The effect of ionic strength of agarose solution and quenching temperature of the emulsion on the structure and mechanical strength of agarose-based chromatographic adsorbents was investigated. Solutions of agarose containing different amounts of NaCl were emulsified at elevated temperature in mineral oil using a high-shear mixer. The hot emulsion was quenched at different temperatures leading to the gelation of agarose and formation of soft particles. Analysis of Atomic Force Microscopy (AFM) images of particle surfaces shows that pore size of particles increases with ionic strength and/or high quenching temperature. Additionally it has been found that the compressive strength of particles measured by micromanipulation also increases with ionic strength of the emulsion and/or high quenching temperature but these two parameters have no significant effect on the resulting particle size and particle size distribution. Results from both characterization methods were compared with Sepharose 4B, a commercial agarose-based adsorbent. This is the first report examining the effect of ionic strength and cooling conditions on the microstructure of micron-sized agarose beads for bioseparation.",
author = "Nicolas Ioannidis and James Bowen and Andrzej Pacek and Zhibing Zhang",
year = "2012",
month = feb,
doi = "10.1016/j.jcis.2011.10.063",
language = "English",
volume = "367",
pages = "153--160",
journal = "Journal of Colloid and Interface Science",
issn = "0021-9797",
publisher = "Elsevier",
number = "1",

}

RIS

TY - JOUR

T1 - Manufacturing of agarose-based chromatographic adsorbents - Effect of ionic strength and cooling conditions on particle structure and mechanical strength.

AU - Ioannidis, Nicolas

AU - Bowen, James

AU - Pacek, Andrzej

AU - Zhang, Zhibing

PY - 2012/2

Y1 - 2012/2

N2 - The effect of ionic strength of agarose solution and quenching temperature of the emulsion on the structure and mechanical strength of agarose-based chromatographic adsorbents was investigated. Solutions of agarose containing different amounts of NaCl were emulsified at elevated temperature in mineral oil using a high-shear mixer. The hot emulsion was quenched at different temperatures leading to the gelation of agarose and formation of soft particles. Analysis of Atomic Force Microscopy (AFM) images of particle surfaces shows that pore size of particles increases with ionic strength and/or high quenching temperature. Additionally it has been found that the compressive strength of particles measured by micromanipulation also increases with ionic strength of the emulsion and/or high quenching temperature but these two parameters have no significant effect on the resulting particle size and particle size distribution. Results from both characterization methods were compared with Sepharose 4B, a commercial agarose-based adsorbent. This is the first report examining the effect of ionic strength and cooling conditions on the microstructure of micron-sized agarose beads for bioseparation.

AB - The effect of ionic strength of agarose solution and quenching temperature of the emulsion on the structure and mechanical strength of agarose-based chromatographic adsorbents was investigated. Solutions of agarose containing different amounts of NaCl were emulsified at elevated temperature in mineral oil using a high-shear mixer. The hot emulsion was quenched at different temperatures leading to the gelation of agarose and formation of soft particles. Analysis of Atomic Force Microscopy (AFM) images of particle surfaces shows that pore size of particles increases with ionic strength and/or high quenching temperature. Additionally it has been found that the compressive strength of particles measured by micromanipulation also increases with ionic strength of the emulsion and/or high quenching temperature but these two parameters have no significant effect on the resulting particle size and particle size distribution. Results from both characterization methods were compared with Sepharose 4B, a commercial agarose-based adsorbent. This is the first report examining the effect of ionic strength and cooling conditions on the microstructure of micron-sized agarose beads for bioseparation.

U2 - 10.1016/j.jcis.2011.10.063

DO - 10.1016/j.jcis.2011.10.063

M3 - Article

C2 - 22115158

VL - 367

SP - 153

EP - 160

JO - Journal of Colloid and Interface Science

JF - Journal of Colloid and Interface Science

SN - 0021-9797

IS - 1

ER -