MAIT cell clonal expansion and TCR repertoire shaping in human volunteers challenged with Salmonella Paratyphi A
Research output: Contribution to journal › Article › peer-review
Colleges, School and Institutes
- University of Oxford
- Immunocore Ltd, 101 Park Drive, Milton Park, Abingdon, OX14 4RY, UK.
- Oxford Vaccine Group, Department of Paediatrics, University of Oxford and the National Institute for Health Research (NIHR) Oxford Biomedical Research Centre, Oxford, OX3 9DU, UK.
- Agency for Science, Technology and Research (A*STAR), Singapore Immunology Network (SIgN), Singapore, 138648, Singapore.
- School of Biosciences, University of Birmingham
Mucosal-associated invariant T (MAIT) cells are innate-like T cells that can detect bacteria-derived metabolites presented on MR1. Here we show, using a controlled infection of humans with live Salmonella enterica serovar Paratyphi A, that MAIT cells are activated during infection, an effect maintained even after antibiotic treatment. At the peak of infection MAIT cell T-cell receptor (TCR)β clonotypes that are over-represented prior to infection transiently contract. Select MAIT cell TCRβ clonotypes that expand after infection have stronger TCR-dependent activation than do contracted clonotypes. Our results demonstrate that host exposure to antigen may drive clonal expansion of MAIT cells with increased functional avidity, suggesting a role for specific vaccination strategies to increase the frequency and potency of MAIT cells to optimize effector function.
|Publication status||Published - 17 Jan 2018|
- Antimicrobial responses, Infection, T-cell receptor