Luminescence screening assays for the identification of sensitizers for lanthanides based on the controlled formation of ternary lanthanide complexes with DTPA-Bisamide Ligands

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@article{100b9836e2504d0ebe2cea988444731c,
title = "Luminescence screening assays for the identification of sensitizers for lanthanides based on the controlled formation of ternary lanthanide complexes with DTPA-Bisamide Ligands",
abstract = "The formation of ternary complexes of lanthanide-diethylenetriamine pentaacetic acid (DTPA)-bisamide complexes with different aromatic acids and their application in luminescent screening assays are presented. The europium complexes of DTPA-bisethylamide (BEA), DTPA-bisbutylamide (BUA), DTPA-bis(2-norbornyl) (NBA), and DTPA-bis(l-adamantyl) (ADA) have been isolated and the sensitization of emission upon 1:1 formation with aromatic acids has been studied by luminescence spectroscopy. The ternary complexes show stronger luminescence with picolinate (PCA) rather than phthalate (PTA) or benzoate (BZA), with the latter forming 1:2 complexes. Isophthalate and dipicolinate sensitizers do not show a 1:1 formation of the tertiary complexes. Electrospray mass spectra show characteristic peaks that confirm the formation of the ternary complexes, and NMR spectroscopic studies demonstrated a conformational locking effect upon formation of the tertiary complex. It is shown that europium complexes with bulkier amide arms provide complexes with stronger luminescence enhancement that is clearly attributable to the bulkiness of the amide arms. In a luminescence screening experiment, different substitutions on BZA and PICA were examined. Sensitizers with a long alkyl chain show greater luminescent enhancements than the nonsubstituted acids, which can be attributed to an {"}umbrella{"} effect of the alkyl chain that protects the europium luminescent center from the quenching of secondary high-energy vibrations, in particular O-H from water molecules. The same effect is presented for the quinoline derivatives as sensitizers. Quinoline derivatives with salicylate binding units were identified as the best sensitizers when combined with EuADA, which is even bulkier than EuNBA, with HQ-3COO showing a 50-fold enhancement of emission upon formation of 1:1 complexes.",
keywords = "luminescence, ligand design, europium, lanthanides, sensitizers",
author = "Ruston, {Linette L.} and Robertson, {Graeme M.} and Zoe Pikramenou",
year = "2010",
month = mar,
day = "1",
doi = "10.1002/asia.200900367",
language = "English",
volume = "5",
pages = "517--580",
journal = "Chemistry - An Asian Journal",
issn = "1861-4728",
publisher = "Wiley-VCH Verlag",
number = "3",

}

RIS

TY - JOUR

T1 - Luminescence screening assays for the identification of sensitizers for lanthanides based on the controlled formation of ternary lanthanide complexes with DTPA-Bisamide Ligands

AU - Ruston, Linette L.

AU - Robertson, Graeme M.

AU - Pikramenou, Zoe

PY - 2010/3/1

Y1 - 2010/3/1

N2 - The formation of ternary complexes of lanthanide-diethylenetriamine pentaacetic acid (DTPA)-bisamide complexes with different aromatic acids and their application in luminescent screening assays are presented. The europium complexes of DTPA-bisethylamide (BEA), DTPA-bisbutylamide (BUA), DTPA-bis(2-norbornyl) (NBA), and DTPA-bis(l-adamantyl) (ADA) have been isolated and the sensitization of emission upon 1:1 formation with aromatic acids has been studied by luminescence spectroscopy. The ternary complexes show stronger luminescence with picolinate (PCA) rather than phthalate (PTA) or benzoate (BZA), with the latter forming 1:2 complexes. Isophthalate and dipicolinate sensitizers do not show a 1:1 formation of the tertiary complexes. Electrospray mass spectra show characteristic peaks that confirm the formation of the ternary complexes, and NMR spectroscopic studies demonstrated a conformational locking effect upon formation of the tertiary complex. It is shown that europium complexes with bulkier amide arms provide complexes with stronger luminescence enhancement that is clearly attributable to the bulkiness of the amide arms. In a luminescence screening experiment, different substitutions on BZA and PICA were examined. Sensitizers with a long alkyl chain show greater luminescent enhancements than the nonsubstituted acids, which can be attributed to an "umbrella" effect of the alkyl chain that protects the europium luminescent center from the quenching of secondary high-energy vibrations, in particular O-H from water molecules. The same effect is presented for the quinoline derivatives as sensitizers. Quinoline derivatives with salicylate binding units were identified as the best sensitizers when combined with EuADA, which is even bulkier than EuNBA, with HQ-3COO showing a 50-fold enhancement of emission upon formation of 1:1 complexes.

AB - The formation of ternary complexes of lanthanide-diethylenetriamine pentaacetic acid (DTPA)-bisamide complexes with different aromatic acids and their application in luminescent screening assays are presented. The europium complexes of DTPA-bisethylamide (BEA), DTPA-bisbutylamide (BUA), DTPA-bis(2-norbornyl) (NBA), and DTPA-bis(l-adamantyl) (ADA) have been isolated and the sensitization of emission upon 1:1 formation with aromatic acids has been studied by luminescence spectroscopy. The ternary complexes show stronger luminescence with picolinate (PCA) rather than phthalate (PTA) or benzoate (BZA), with the latter forming 1:2 complexes. Isophthalate and dipicolinate sensitizers do not show a 1:1 formation of the tertiary complexes. Electrospray mass spectra show characteristic peaks that confirm the formation of the ternary complexes, and NMR spectroscopic studies demonstrated a conformational locking effect upon formation of the tertiary complex. It is shown that europium complexes with bulkier amide arms provide complexes with stronger luminescence enhancement that is clearly attributable to the bulkiness of the amide arms. In a luminescence screening experiment, different substitutions on BZA and PICA were examined. Sensitizers with a long alkyl chain show greater luminescent enhancements than the nonsubstituted acids, which can be attributed to an "umbrella" effect of the alkyl chain that protects the europium luminescent center from the quenching of secondary high-energy vibrations, in particular O-H from water molecules. The same effect is presented for the quinoline derivatives as sensitizers. Quinoline derivatives with salicylate binding units were identified as the best sensitizers when combined with EuADA, which is even bulkier than EuNBA, with HQ-3COO showing a 50-fold enhancement of emission upon formation of 1:1 complexes.

KW - luminescence

KW - ligand design

KW - europium

KW - lanthanides

KW - sensitizers

UR - http://www.scopus.com/inward/record.url?eid=2-s2.0-77749339913&partnerID=MN8TOARS

U2 - 10.1002/asia.200900367

DO - 10.1002/asia.200900367

M3 - Article

C2 - 20014090

VL - 5

SP - 517

EP - 580

JO - Chemistry - An Asian Journal

JF - Chemistry - An Asian Journal

SN - 1861-4728

IS - 3

ER -