Localisation of annexins in the retina of the rainbow trout - light and electron microscopical investigations

We present a first description of annexin immunoreactivity within the teleost retina. Antibodies against annexins V and VI were used in light and electron microscopic sections of light- and dark-adapted retinae. Strong immunoreactivity could be found in retinal layers with high synaptic input, such as the outer and inner plexiform layers and dendritic regions within the inner plexiform layer, in cells that are involved in negative feedback control such as horizontal and amacrine cells, in the membrane metabolism of photoreceptor outer segments, and in close relation to cytoskeletal components. Our findings suggest that both annexins V and VI are involved in the regulation of transmitter release, particularly of transmitters that are not directly involved in phototransduction. The annexins appear also to be involved with structures that support morphological changes in light and dark adaptation.