LNGFR+THY-1+VCAM-1hi+ cells reveal functionally distinct subpopulations in mesenchymal stem cells

Research output: Contribution to journalArticlepeer-review


  • Yo Mabuchi
  • Satoru Morikawa
  • Seiko Harada
  • Kunimichi Niibe
  • Sadafumi Suzuki
  • Francois Renault-mihara
  • Chihiro Akazawa
  • Hideyuki Okano
  • Yumi Matsuzaki

Colleges, School and Institutes


Human mesenchymal stem cells (hMSCs), which conventionally are isolated based on their adherence to plastic, are heterogeneous and have poor growth and differentiation, limiting our ability to investigate their intrinsic characteristics. We report an improved prospective clonal isolation technique and reveal that the combination of three cell-surface markers (LNGFR, THY-1, and VCAM-1) allows for the selection of highly enriched clonogenic cells (one out of three isolated cells). Clonal characterization of LNGFR+THY-1+ cells demonstrated cellular heterogeneity among the clones. Rapidly expanding clones (RECs) exhibited robust multilineage differentiation and self-renewal potency, whereas the other clones tended to acquire cellular senescence via P16INK4a and exhibited frequent genomic errors. Furthermore, RECs exhibited unique expression of VCAM-1 and higher cellular motility compared with the other clones. The combination marker LNGFR+THY-1+VCAM-1hi+ (LTV) can be used selectively to isolate the most potent and genetically stable MSCs.


Original languageEnglish
Pages (from-to)152-165
JournalStem Cell Reports
Issue number2
Early online date11 Jul 2013
Publication statusPublished - 6 Aug 2013