Projects per year
Abstract
Outer membrane vesicles are nano-sized microvesicles shed from the outer membrane of Gram-negative bacteria and play important roles in immune priming and disease pathogenesis. However, our current mechanistic understanding of vesicle - host cell interactions is limited by a lack of methods to study the rapid kinetics of vesicle entry and cargo delivery to host cells. Here, we describe a highly sensitive method to study the kinetics of vesicle entry into host cells in real-time using a genetically encoded, vesicle-targeted probe. We found that the route of vesicular uptake, and thus entry kinetics and efficiency, are shaped by bacterial cell wall composition. The presence of lipopolysaccharide O antigen enables vesicles to bypass clathrin-mediated endocytosis, which enhances both their entry rate and efficiency into host cells. Collectively, our findings highlight the composition of the bacterial cell wall as a major determinant of secretion-independent delivery of virulence factors during Gram-negative infections.
Original language | English |
---|---|
Pages (from-to) | e1006760 |
Journal | PLoS pathogens |
Volume | 13 |
Issue number | 11 |
DOIs | |
Publication status | Published - 29 Nov 2017 |
Keywords
- outer membrane vesicles
- lipopolysaccharide
- serotype
- FRET kinetics
- extracellular vesicles
- cargo uptake
- vesicle trafficking
- endocytosis
Fingerprint
Dive into the research topics of 'Lipopolysaccharide structure impacts the entry kinetics of bacterial outer membrane vesicles into host cells'. Together they form a unique fingerprint.Projects
- 1 Finished
-
Molecular and functional characterization of protein-lipid interactions at the bacterial host interface
Krachler, A.
Biotechnology & Biological Sciences Research Council
1/04/14 → 31/03/17
Project: Research Councils