Linear dichroism of visible-region chromophores using M13 bacteriophage as an alignment scaffold

Research output: Contribution to journalArticle

Standard

Linear dichroism of visible-region chromophores using M13 bacteriophage as an alignment scaffold. / Tridgett, Matthew; Moore-kelly, Charles; Duprey, Jean-louis H. A.; Iturbe, Lorea Orueta; Tsang, Chi w.; Little, Haydn A.; Sandhu, Sandeep K.; Hicks, Matthew R.; Dafforn, Timothy R.; Rodger, Alison.

In: RSC Advances, Vol. 8, No. 52, 20.08.2018, p. 29535-29543.

Research output: Contribution to journalArticle

Harvard

Tridgett, M, Moore-kelly, C, Duprey, JHA, Iturbe, LO, Tsang, C, Little, HA, Sandhu, SK, Hicks, MR, Dafforn, TR & Rodger, A 2018, 'Linear dichroism of visible-region chromophores using M13 bacteriophage as an alignment scaffold', RSC Advances, vol. 8, no. 52, pp. 29535-29543. https://doi.org/10.1039/C8RA05475D

APA

Tridgett, M., Moore-kelly, C., Duprey, J. H. A., Iturbe, L. O., Tsang, C., Little, H. A., Sandhu, S. K., Hicks, M. R., Dafforn, T. R., & Rodger, A. (2018). Linear dichroism of visible-region chromophores using M13 bacteriophage as an alignment scaffold. RSC Advances, 8(52), 29535-29543. https://doi.org/10.1039/C8RA05475D

Vancouver

Author

Tridgett, Matthew ; Moore-kelly, Charles ; Duprey, Jean-louis H. A. ; Iturbe, Lorea Orueta ; Tsang, Chi w. ; Little, Haydn A. ; Sandhu, Sandeep K. ; Hicks, Matthew R. ; Dafforn, Timothy R. ; Rodger, Alison. / Linear dichroism of visible-region chromophores using M13 bacteriophage as an alignment scaffold. In: RSC Advances. 2018 ; Vol. 8, No. 52. pp. 29535-29543.

Bibtex

@article{0532fe17148c4d3c8876eda630508d04,
title = "Linear dichroism of visible-region chromophores using M13 bacteriophage as an alignment scaffold",
abstract = "It is a challenge within the field of biomimetics to recreate the properties of light-harvesting antennae found in plants and photosynthetic bacteria. Attempts to recreate these biological structures typically rely on the alignment of fluorescent moieties via attachment to an inert linear scaffold, e.g. DNA, RNA or amyloid fibrils, to enable F{\"o}rster resonance energy transfer (FRET) between attached chromophores. While there has been some success in this approach, refinement of the alignment of the chromophores is often limited, which may limit the efficiency of energy transfer achieved. Here we demonstrate how linear dichroism spectroscopy may be used to ascertain the overall alignment of chromophores bound to the M13 bacteriophage, a model linear scaffold, and demonstrate how this may be used to distinguish between lack of FRET efficiency due to chromophore separation, and chromophore misalignment. This approach will allow the refinement of artificial light-harvesting antennae in a directed fashion.",
author = "Matthew Tridgett and Charles Moore-kelly and Duprey, {Jean-louis H. A.} and Iturbe, {Lorea Orueta} and Chi w. Tsang and Little, {Haydn A.} and Sandhu, {Sandeep K.} and Hicks, {Matthew R.} and Dafforn, {Timothy R.} and Alison Rodger",
year = "2018",
month = aug,
day = "20",
doi = "10.1039/C8RA05475D",
language = "English",
volume = "8",
pages = "29535--29543",
journal = "RSC Advances",
issn = "2046-2069",
publisher = "Royal Society of Chemistry",
number = "52",

}

RIS

TY - JOUR

T1 - Linear dichroism of visible-region chromophores using M13 bacteriophage as an alignment scaffold

AU - Tridgett, Matthew

AU - Moore-kelly, Charles

AU - Duprey, Jean-louis H. A.

AU - Iturbe, Lorea Orueta

AU - Tsang, Chi w.

AU - Little, Haydn A.

AU - Sandhu, Sandeep K.

AU - Hicks, Matthew R.

AU - Dafforn, Timothy R.

AU - Rodger, Alison

PY - 2018/8/20

Y1 - 2018/8/20

N2 - It is a challenge within the field of biomimetics to recreate the properties of light-harvesting antennae found in plants and photosynthetic bacteria. Attempts to recreate these biological structures typically rely on the alignment of fluorescent moieties via attachment to an inert linear scaffold, e.g. DNA, RNA or amyloid fibrils, to enable Förster resonance energy transfer (FRET) between attached chromophores. While there has been some success in this approach, refinement of the alignment of the chromophores is often limited, which may limit the efficiency of energy transfer achieved. Here we demonstrate how linear dichroism spectroscopy may be used to ascertain the overall alignment of chromophores bound to the M13 bacteriophage, a model linear scaffold, and demonstrate how this may be used to distinguish between lack of FRET efficiency due to chromophore separation, and chromophore misalignment. This approach will allow the refinement of artificial light-harvesting antennae in a directed fashion.

AB - It is a challenge within the field of biomimetics to recreate the properties of light-harvesting antennae found in plants and photosynthetic bacteria. Attempts to recreate these biological structures typically rely on the alignment of fluorescent moieties via attachment to an inert linear scaffold, e.g. DNA, RNA or amyloid fibrils, to enable Förster resonance energy transfer (FRET) between attached chromophores. While there has been some success in this approach, refinement of the alignment of the chromophores is often limited, which may limit the efficiency of energy transfer achieved. Here we demonstrate how linear dichroism spectroscopy may be used to ascertain the overall alignment of chromophores bound to the M13 bacteriophage, a model linear scaffold, and demonstrate how this may be used to distinguish between lack of FRET efficiency due to chromophore separation, and chromophore misalignment. This approach will allow the refinement of artificial light-harvesting antennae in a directed fashion.

U2 - 10.1039/C8RA05475D

DO - 10.1039/C8RA05475D

M3 - Article

VL - 8

SP - 29535

EP - 29543

JO - RSC Advances

JF - RSC Advances

SN - 2046-2069

IS - 52

ER -