TY - JOUR
T1 - Length-dependent retention of carbon nanotubes in the pleural space of mice initiates sustained inflammation and progressive fibrosis on the parietal pleura
AU - Murphy, Fiona A
AU - Poland, Craig A
AU - Duffin, Rodger
AU - Al-Jamal, Khuloud T
AU - Ali-Boucetta, Hanene
AU - Nunes, Antonio
AU - Byrne, Fiona
AU - Prina-Mello, Adriele
AU - Volkov, Yuri
AU - Li, Shouping
AU - Mather, Stephen J
AU - Bianco, Alberto
AU - Prato, Maurizio
AU - Macnee, William
AU - Wallace, William A
AU - Kostarelos, Kostas
AU - Donaldson, Ken
N1 - Copyright © 2011 American Society for Investigative Pathology. Published by Elsevier Inc. All rights reserved.
PY - 2011/6
Y1 - 2011/6
N2 - The fibrous shape of carbon nanotubes (CNTs) raises concern that they may pose an asbestos-like inhalation hazard, leading to the development of diseases, especially mesothelioma. Direct instillation of long and short CNTs into the pleural cavity, the site of mesothelioma development, produced asbestos-like length-dependent responses. The response to long CNTs and long asbestos was characterized by acute inflammation, leading to progressive fibrosis on the parietal pleura, where stomata of strictly defined size limit the egress of long, but not short, fibers. This was confirmed by demonstrating clearance of short, but not long, CNT and nickel nanowires and by visualizing the migration of short CNTs from the pleural space by single-photon emission computed tomographic imaging. Our data confirm the hypothesis that, although a proportion of all deposited particles passes through the pleura, the pathogenicity of long CNTs and other fibers arises as a result of length-dependent retention at the stomata on the parietal pleura.
AB - The fibrous shape of carbon nanotubes (CNTs) raises concern that they may pose an asbestos-like inhalation hazard, leading to the development of diseases, especially mesothelioma. Direct instillation of long and short CNTs into the pleural cavity, the site of mesothelioma development, produced asbestos-like length-dependent responses. The response to long CNTs and long asbestos was characterized by acute inflammation, leading to progressive fibrosis on the parietal pleura, where stomata of strictly defined size limit the egress of long, but not short, fibers. This was confirmed by demonstrating clearance of short, but not long, CNT and nickel nanowires and by visualizing the migration of short CNTs from the pleural space by single-photon emission computed tomographic imaging. Our data confirm the hypothesis that, although a proportion of all deposited particles passes through the pleura, the pathogenicity of long CNTs and other fibers arises as a result of length-dependent retention at the stomata on the parietal pleura.
KW - Animals
KW - Cell Proliferation
KW - Disease Progression
KW - Epithelium/pathology
KW - Fibrosis
KW - Inflammation/complications
KW - Lymph Nodes/pathology
KW - Mediastinum/pathology
KW - Mice
KW - Nanotubes, Carbon/chemistry
KW - Nanowires/ultrastructure
KW - Particle Size
KW - Pleura/pathology
KW - Pleural Cavity/pathology
KW - Time Factors
KW - Tomography, Emission-Computed, Single-Photon
KW - Tomography, X-Ray Computed
U2 - 10.1016/j.ajpath.2011.02.040
DO - 10.1016/j.ajpath.2011.02.040
M3 - Article
C2 - 21641383
SN - 0002-9440
VL - 178
SP - 2587
EP - 2600
JO - The American Journal of Pathology
JF - The American Journal of Pathology
IS - 6
ER -