Leg glucose and protein metabolism during an acute bout of resistance exercise in humans

Research output: Contribution to journalArticle


  • WJ Durham
  • SL Miller
  • CW Yeckel
  • DL Chinkes
  • BB Rasmussen
  • RR Wolfe

Colleges, School and Institutes


The present study investigated the responses of leg glucose and protein metabolism during an acute bout of resistance exercise. Seven subjects (5 men, 2 women) were studied at rest and during a strenuous lower body resistance exercise regimen consisting of approximately 8 sets of 10 repetitions of leg press at approximately 75% 1 repetition maximum and 8 sets of 8 repetitions of knee extensions at approximately 80% 1 repetition maximum. L-[ring-2H5]phenylalanine was infused throughout the study for measurement of phenylalanine rates of appearance, disappearance, protein synthesis, and protein breakdown across the leg. Femoral arterial and venous blood samples were collected at rest and during exercise for determination of leg blood flow, concentrations of glucose, lactate, alanine, glutamine, glutamate, leucine, and phenylalanine, and phenylalanine enrichments. Muscle biopsies were obtained at rest and immediately after exercise. Leg blood flow was nearly three times (P


Original languageEnglish
Pages (from-to)1379-1386
Number of pages8
JournalJournal of Applied Physiology
Publication statusPublished - 14 May 2004