LARP1 isoform expression in human cancer cell lines

Research output: Contribution to journalArticlepeer-review

Authors

  • Hagen Schwenzer
  • Mai Abdel Mouti
  • Pia Neubert
  • Josephine Morris
  • Sarah Bonham
  • Martin Fellermeyer
  • James Chettle
  • Roman Fischer
  • Sarah P Blagden

Colleges, School and Institutes

Abstract

LARP1 is an oncogenic RNA-binding protein required for ribosome biogenesis and cancer cell survival. From published in vitro studies, there is disparity over which of two different LARP1 protein isoforms (termed the long LI-LARP1 and short SI-LARP1) is the canonical. Here, after conducting a series of biochemical and cellular assays, we conclude that LI-LARP1 (NM_033551.3 > NP_056130.2) is the dominantly expressed form. We observe that SI-LARP1 (NM_015315.5> NP_056130.2) is epigenetically repressed and that this repression is evolutionarily conserved in all but a small subclade of mammalian species. As with other LARP family members, there are multiple potential LARP1 mRNA isoforms that appear to be censored within the nucleus. The capacity of the cell to modulate splicing and expression of these apparently 'redundant' mRNAs hints at contextually specific mechanisms of LARP1 expression.

Details

Original languageEnglish
Number of pages11
JournalRNA biology
Early online date14 Apr 2020
Publication statusE-pub ahead of print - 14 Apr 2020

Keywords

  • LARP1, RNA binding proteins, cancer, alternative protein isoforms