Kaposi's sarcoma-associated herpesvirus viral interferon regulatory factor-2 inhibits type 1 interferon signalling by targeting interferon-stimulated gene factor-3.

Research output: Contribution to journalArticle

Authors

  • Mohamed Mutocheluh
  • Laura Hindle
  • C Aresté
  • Lucilla Butler
  • K Lowry
  • K Shah
  • DJ Evans

Abstract

Kaposi's sarcoma-associated herpesvirus (KSHV) encodes four viral interferon regulatory factors (vIRF-1-4). We investigated the mechanism and consequences of vIRF-2-mediated inhibition of interferon-response element signalling following type I interferon (IFN) induction. Western blot and electrophoretic mobility-shift assays identified the interferon-stimulated gene factor-3 (ISGF-3) components STAT1 and IRF-9 as the proximal targets of vIRF-2 activity. The biological significance of vIRF-2 inhibition of ISGF-3 was demonstrated by vIRF-2-mediated rescue of the replication of the IFN-sensitive virus encephalomyocarditis virus. This study provides both a mechanism and evidence for KSHV vIRF-2-mediated suppression of the consequences of type 1 IFN-induced signalling.

Details

Original languageEnglish
Pages (from-to)2394-8
Number of pages5
JournalJournal of General Virology
Volume92
Issue numberPt 10
Publication statusPublished - 1 Oct 2011