Abstract
The interaction between the HIV-1 transactivator protein Tat and TAR (transactivation responsive region) RNA, plays a critical role in HIV-1 transcription. Iron(II) supramolecular helicates were evaluated for their in vitro activity to inhibit Tat–TAR RNA interaction using UV melting studies, electrophoretic mobility shift assay, and RNase A footprinting. The results demonstrate that iron(II) supramolecular helicates inhibit Tat-TAR interaction at nanomolar concentrations by binding to TAR RNA. These studies provide a new insight into the biological potential of metallosupramolecular helicates.
Original language | English |
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Article number | 29674 |
Journal | Scientific Reports |
Volume | 6 |
Early online date | 12 Jul 2016 |
DOIs | |
Publication status | Published - 2016 |
Keywords
- RNA
- Self-assembly