Investigation of the syncytial nature of detrusor smooth muscle as a determinant of action potential shape

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Investigation of the syncytial nature of detrusor smooth muscle as a determinant of action potential shape. / Appukuttan, Shailesh; Padmakumar, Mithun; Young, John; Brain, Keith; Manchanda, Rohit.

In: Frontiers in Physiology, Vol. 9, 1300, 20.09.2018.

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Appukuttan, Shailesh ; Padmakumar, Mithun ; Young, John ; Brain, Keith ; Manchanda, Rohit. / Investigation of the syncytial nature of detrusor smooth muscle as a determinant of action potential shape. In: Frontiers in Physiology. 2018 ; Vol. 9.

Bibtex

@article{44257443541f4f2391cd9cdc19bb9bf7,
title = "Investigation of the syncytial nature of detrusor smooth muscle as a determinant of action potential shape",
abstract = "Unlike most excitable cells, certain syncytial smooth muscle cells are known to exhibit spontaneous action potentials of varying shapes and sizes. These differences in shape are observed even in electrophysiological recordings obtained from a single cell. The origin and physiological relevance of this phenomenon are currently unclear. The study presented here aims to test the hypothesis that the syncytial nature of the detrusor smooth muscle tissue contributes to the variations in the action potential profile by influencing the superposition of thepassive and active signals. Data extracted from experimental recordings have been compared with those obtained through simulations. The feature correlation studies on action potentials obtained from the experimental recordings suggest the underlying presence of passive signals, called spontaneous excitatory junction potentials (sEJPs). Through simulations, we are able to demonstrate that the syncytial organization of the cells, and the variable superposition of the sEJPs with the {\textquoteleft}native action potential{\textquoteright}, contribute to the diversity in the action potential profilesexhibited. It could also be inferred that the fraction of the propagated action potentials is very low in the detrusor. It is proposed that objective measurements of spontaneous action potential profiles can lead to a better understanding of bladder physiology and pathology.",
keywords = "bladder, smooth muscle cells, Detrusor overactivity, Action potentials, Feature extraction, NEURON",
author = "Shailesh Appukuttan and Mithun Padmakumar and John Young and Keith Brain and Rohit Manchanda",
year = "2018",
month = sep,
day = "20",
doi = "10.3389/fphys.2018.01300",
language = "English",
volume = "9",
journal = "Frontiers in Physiology",
issn = "1664-042X",
publisher = "Frontiers",

}

RIS

TY - JOUR

T1 - Investigation of the syncytial nature of detrusor smooth muscle as a determinant of action potential shape

AU - Appukuttan, Shailesh

AU - Padmakumar, Mithun

AU - Young, John

AU - Brain, Keith

AU - Manchanda, Rohit

PY - 2018/9/20

Y1 - 2018/9/20

N2 - Unlike most excitable cells, certain syncytial smooth muscle cells are known to exhibit spontaneous action potentials of varying shapes and sizes. These differences in shape are observed even in electrophysiological recordings obtained from a single cell. The origin and physiological relevance of this phenomenon are currently unclear. The study presented here aims to test the hypothesis that the syncytial nature of the detrusor smooth muscle tissue contributes to the variations in the action potential profile by influencing the superposition of thepassive and active signals. Data extracted from experimental recordings have been compared with those obtained through simulations. The feature correlation studies on action potentials obtained from the experimental recordings suggest the underlying presence of passive signals, called spontaneous excitatory junction potentials (sEJPs). Through simulations, we are able to demonstrate that the syncytial organization of the cells, and the variable superposition of the sEJPs with the ‘native action potential’, contribute to the diversity in the action potential profilesexhibited. It could also be inferred that the fraction of the propagated action potentials is very low in the detrusor. It is proposed that objective measurements of spontaneous action potential profiles can lead to a better understanding of bladder physiology and pathology.

AB - Unlike most excitable cells, certain syncytial smooth muscle cells are known to exhibit spontaneous action potentials of varying shapes and sizes. These differences in shape are observed even in electrophysiological recordings obtained from a single cell. The origin and physiological relevance of this phenomenon are currently unclear. The study presented here aims to test the hypothesis that the syncytial nature of the detrusor smooth muscle tissue contributes to the variations in the action potential profile by influencing the superposition of thepassive and active signals. Data extracted from experimental recordings have been compared with those obtained through simulations. The feature correlation studies on action potentials obtained from the experimental recordings suggest the underlying presence of passive signals, called spontaneous excitatory junction potentials (sEJPs). Through simulations, we are able to demonstrate that the syncytial organization of the cells, and the variable superposition of the sEJPs with the ‘native action potential’, contribute to the diversity in the action potential profilesexhibited. It could also be inferred that the fraction of the propagated action potentials is very low in the detrusor. It is proposed that objective measurements of spontaneous action potential profiles can lead to a better understanding of bladder physiology and pathology.

KW - bladder

KW - smooth muscle cells

KW - Detrusor overactivity

KW - Action potentials

KW - Feature extraction

KW - NEURON

U2 - 10.3389/fphys.2018.01300

DO - 10.3389/fphys.2018.01300

M3 - Article

C2 - 30294280

VL - 9

JO - Frontiers in Physiology

JF - Frontiers in Physiology

SN - 1664-042X

M1 - 1300

ER -