Intramolecular DNA coiling mediated by metallo-supramolecular cylinders: differential binding of P and M helical enantiomers

Research output: Contribution to journalArticle

Authors

  • I Meistermann
  • V Moreno
  • MJ Prieto
  • E Molderheim
  • E Sletten
  • S Khalid
  • PM Rodger
  • JC Peberdy
  • CJ Isaac
  • A Rodger

Colleges, School and Institutes

Abstract

We have designed a synthetic tetracationic metallo-supramolecular cylinder that targets the major groove of DNA with a binding constant in excess of 10(7) M(-1) and induces DNA bending and intramolecular coiling. The two enantiomers of the helical molecule bind differently to DNA and have different structural effects. We report the characterization of the interactions by a range of biophysical techniques. The M helical cylinder binds to the major groove and induces dramatic intramolecular coiling. The DNA bending is less dramatic for the P enantiomer.

Details

Original languageEnglish
Pages (from-to)5069-5074
Number of pages6
JournalNational Academy of Sciences. Proceedings
Volume99
Publication statusPublished - 1 Jan 2002