Interfacial partition of plasmid DNA in aqueous two-phase systems

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Interfacial partition of plasmid DNA in aqueous two-phase systems. / Luechau, F; Ling, TC; Lyddiatt, Andrew.

In: Separation and Purification Technology, Vol. 67, No. 1, 01.05.2009, p. 64-70.

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@article{37f09249f94f420cb1a864514e966f3d,
title = "Interfacial partition of plasmid DNA in aqueous two-phase systems",
abstract = "In this paper, the partition of plasmid DNA (pDNA) in polyethylene glycol (PEG) 300 and di-potassium hydrogen phosphate aqueous two-phase systems (ATPS) under gravity separation was investigated. The influence of temperature (20 and 30 degrees C) and mode of phase separation (40-60% (w/w) lysate concentrations) on the pDNA partition behaviour was studied. At 20 degrees C, 70.7% of pDNA partitioned to the top phase while the recoveries in the interface and bottom phase were comparatively small. At 30 degrees C, the partition had switched from a top phase preference to an interface preference where 73.9% of pDNA was recovered. The distribution of pDNA between the top phase and interface is effectively time-dependent during gravitational phase separation. Systems with lysate concentrations between 40 and 50% (w/w) separated with a continuous bottom phase and a dispersed PEG-rich top phase. For ATPS with 55 and 60% (w/w) lysate, the top phase was continuous and the bottom phase was dispersed. (C) 2009 Elsevier B.V. All rights reserved.",
keywords = "Interfacial partition, Top phase, Aqueous two-phase systems, Plasmid DNA, Bottom phase",
author = "F Luechau and TC Ling and Andrew Lyddiatt",
year = "2009",
month = may,
day = "1",
doi = "10.1016/j.seppur.2009.03.027",
language = "English",
volume = "67",
pages = "64--70",
journal = "Separation and Purification Technology",
issn = "1383-5866",
publisher = "Elsevier",
number = "1",

}

RIS

TY - JOUR

T1 - Interfacial partition of plasmid DNA in aqueous two-phase systems

AU - Luechau, F

AU - Ling, TC

AU - Lyddiatt, Andrew

PY - 2009/5/1

Y1 - 2009/5/1

N2 - In this paper, the partition of plasmid DNA (pDNA) in polyethylene glycol (PEG) 300 and di-potassium hydrogen phosphate aqueous two-phase systems (ATPS) under gravity separation was investigated. The influence of temperature (20 and 30 degrees C) and mode of phase separation (40-60% (w/w) lysate concentrations) on the pDNA partition behaviour was studied. At 20 degrees C, 70.7% of pDNA partitioned to the top phase while the recoveries in the interface and bottom phase were comparatively small. At 30 degrees C, the partition had switched from a top phase preference to an interface preference where 73.9% of pDNA was recovered. The distribution of pDNA between the top phase and interface is effectively time-dependent during gravitational phase separation. Systems with lysate concentrations between 40 and 50% (w/w) separated with a continuous bottom phase and a dispersed PEG-rich top phase. For ATPS with 55 and 60% (w/w) lysate, the top phase was continuous and the bottom phase was dispersed. (C) 2009 Elsevier B.V. All rights reserved.

AB - In this paper, the partition of plasmid DNA (pDNA) in polyethylene glycol (PEG) 300 and di-potassium hydrogen phosphate aqueous two-phase systems (ATPS) under gravity separation was investigated. The influence of temperature (20 and 30 degrees C) and mode of phase separation (40-60% (w/w) lysate concentrations) on the pDNA partition behaviour was studied. At 20 degrees C, 70.7% of pDNA partitioned to the top phase while the recoveries in the interface and bottom phase were comparatively small. At 30 degrees C, the partition had switched from a top phase preference to an interface preference where 73.9% of pDNA was recovered. The distribution of pDNA between the top phase and interface is effectively time-dependent during gravitational phase separation. Systems with lysate concentrations between 40 and 50% (w/w) separated with a continuous bottom phase and a dispersed PEG-rich top phase. For ATPS with 55 and 60% (w/w) lysate, the top phase was continuous and the bottom phase was dispersed. (C) 2009 Elsevier B.V. All rights reserved.

KW - Interfacial partition

KW - Top phase

KW - Aqueous two-phase systems

KW - Plasmid DNA

KW - Bottom phase

U2 - 10.1016/j.seppur.2009.03.027

DO - 10.1016/j.seppur.2009.03.027

M3 - Article

VL - 67

SP - 64

EP - 70

JO - Separation and Purification Technology

JF - Separation and Purification Technology

SN - 1383-5866

IS - 1

ER -