Integrated fluidized bed affinity recovery of an anti-MUC1 mucin recombinant diabody from Escherichia coli lysates

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Integrated fluidized bed affinity recovery of an anti-MUC1 mucin recombinant diabody from Escherichia coli lysates. / Lan, JCW; Ling, TC; O'Sullivan, DA; Lyddiatt, Andrew.

In: Separation and Purification Technology, Vol. 83, 01.11.2011, p. 204-207.

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@article{937f91f9d1054b20a3164c10648d32d3,
title = "Integrated fluidized bed affinity recovery of an anti-MUC1 mucin recombinant diabody from Escherichia coli lysates",
abstract = "The fluidized bed affinity recovery of C595 diabody fragment (dbFv) from Escherichia coli lysates in an integrated system mode has been investigated. An amino acid epitope sequence, RPAP was immobilized onto Upfront adsorbent (rho = 1.35 g cm(-3)). The recovery performance of various feedstocks (clarified, unclarified and concentrated unclarified feedstocks) were compared and evaluated. It was demonstrated that the values of operational productivity for clarified and unclarified feedstock were 198.18 and 129.11 mg L-1, respectively. A relatively high elution yield (87-93%) was achieved for all types of feedstock evaluated. (C) 2011 Elsevier BM. All rights reserved.",
keywords = "Fluidized bed, Integrated system, C595 diabody fragment, Operational productivity, Feedstock",
author = "JCW Lan and TC Ling and DA O'Sullivan and Andrew Lyddiatt",
year = "2011",
month = nov,
day = "1",
doi = "10.1016/j.seppur.2011.09.008",
language = "English",
volume = "83",
pages = "204--207",
journal = "Separation and Purification Technology",
issn = "1383-5866",
publisher = "Elsevier",

}

RIS

TY - JOUR

T1 - Integrated fluidized bed affinity recovery of an anti-MUC1 mucin recombinant diabody from Escherichia coli lysates

AU - Lan, JCW

AU - Ling, TC

AU - O'Sullivan, DA

AU - Lyddiatt, Andrew

PY - 2011/11/1

Y1 - 2011/11/1

N2 - The fluidized bed affinity recovery of C595 diabody fragment (dbFv) from Escherichia coli lysates in an integrated system mode has been investigated. An amino acid epitope sequence, RPAP was immobilized onto Upfront adsorbent (rho = 1.35 g cm(-3)). The recovery performance of various feedstocks (clarified, unclarified and concentrated unclarified feedstocks) were compared and evaluated. It was demonstrated that the values of operational productivity for clarified and unclarified feedstock were 198.18 and 129.11 mg L-1, respectively. A relatively high elution yield (87-93%) was achieved for all types of feedstock evaluated. (C) 2011 Elsevier BM. All rights reserved.

AB - The fluidized bed affinity recovery of C595 diabody fragment (dbFv) from Escherichia coli lysates in an integrated system mode has been investigated. An amino acid epitope sequence, RPAP was immobilized onto Upfront adsorbent (rho = 1.35 g cm(-3)). The recovery performance of various feedstocks (clarified, unclarified and concentrated unclarified feedstocks) were compared and evaluated. It was demonstrated that the values of operational productivity for clarified and unclarified feedstock were 198.18 and 129.11 mg L-1, respectively. A relatively high elution yield (87-93%) was achieved for all types of feedstock evaluated. (C) 2011 Elsevier BM. All rights reserved.

KW - Fluidized bed

KW - Integrated system

KW - C595 diabody fragment

KW - Operational productivity

KW - Feedstock

U2 - 10.1016/j.seppur.2011.09.008

DO - 10.1016/j.seppur.2011.09.008

M3 - Article

VL - 83

SP - 204

EP - 207

JO - Separation and Purification Technology

JF - Separation and Purification Technology

SN - 1383-5866

ER -