Integrated fluidized bed affinity recovery of an anti-MUC1 mucin recombinant diabody from Escherichia coli lysates

JCW Lan, TC Ling, DA O'Sullivan, Andrew Lyddiatt

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

The fluidized bed affinity recovery of C595 diabody fragment (dbFv) from Escherichia coli lysates in an integrated system mode has been investigated. An amino acid epitope sequence, RPAP was immobilized onto Upfront adsorbent (rho = 1.35 g cm(-3)). The recovery performance of various feedstocks (clarified, unclarified and concentrated unclarified feedstocks) were compared and evaluated. It was demonstrated that the values of operational productivity for clarified and unclarified feedstock were 198.18 and 129.11 mg L-1, respectively. A relatively high elution yield (87-93%) was achieved for all types of feedstock evaluated. (C) 2011 Elsevier BM. All rights reserved.
Original languageEnglish
Pages (from-to)204-207
Number of pages4
JournalSeparation and Purification Technology
Volume83
DOIs
Publication statusPublished - 1 Nov 2011

Keywords

  • Fluidized bed
  • Integrated system
  • C595 diabody fragment
  • Operational productivity
  • Feedstock

Fingerprint

Dive into the research topics of 'Integrated fluidized bed affinity recovery of an anti-MUC1 mucin recombinant diabody from Escherichia coli lysates'. Together they form a unique fingerprint.

Cite this