Integrated Analysis of Intracellular Dynamics of MenaINV Cancer Cells in a 3D Matrix
Research output: Contribution to journal › Article
Colleges, School and Institutes
- Harvey Mudd College
- Department of Biomedical Engineering, Yale University
- Department of Mechanical Engineering, Massachusetts Institute of Technology
- Department of Biomedical Engineering, Boston University, Boston MA 02215.
- Howard Hughes Medical Institute, Boston University
The intracellular environment is composed of a filamentous network that exhibits dynamic turnover of cytoskeletal components and internal force generation from molecular motors. Particle tracking microrheology enables a means to probe the internal mechanics and dynamics. Here, we develop an analytical model to capture the basic features of the active intracellular mechanical environment, including both thermal and motor-driven effects, and show consistency with a diverse range of experimental microrheology data. We further perform microrheology experiments, integrated with Brownian dynamics simulations of the active cytoskeleton, on metastatic breast cancer cells embedded in a three-dimensional collagen matrix with and without the presence of epidermal growth factor to probe the intracellular mechanical response in a physiologically mimicking scenario. Our results demonstrate that EGF stimulation can alter intracellular stiffness and power output from molecular motor-driven fluctuations in cells overexpressing an invasive isoform of the actin-associated protein Mena.
|Number of pages||11|
|Publication status||Published - 9 May 2017|