Abstract
Glycogen synthesis increases after muscle contraction and during insulin stimulation, and insulin-stimulated glycogen synthesis is enhanced after contraction. We hypothesized that the initial glycogen content influences the magnitude of additive effect of contraction on insulin-stimulated glycogen synthesis. Contraction and insulin had full additive effect on rate of glycogen synthesis measured after contraction in muscles with normal and high glycogen content. In muscles with low glycogen, contraction increased insulin-stimulated glycogen synthesis nearly as much as in muscles with normal glycogen, but not to the sum of the two stimuli studied separately; still glycogen synthesis was generally highest in muscles with low glycogen. Glycogen synthase fractional activity inversely correlated with glycogen content and contraction increased glycogen synthase fractional activity. Contraction and insulin additively increased glycogen synthase fractional activity at all glycogen contents. In conclusion, after contraction insulin-stimulated glycogen synthesis was increased by rather similar magnitude at all glycogen contents in concert with increased glycogen synthase activation.
Original language | English |
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Pages (from-to) | 116-127 |
Number of pages | 12 |
Journal | Archives of Physiology and Biochemistry |
Volume | 116 |
Issue number | 3 |
DOIs | |
Publication status | Published - 1 Jul 2010 |
Keywords
- Affinity
- Enzyme kinetic
- GSK-3
- P38MAP-kinase
- Phosphorylation
- PKB
- UDP-glucose
ASJC Scopus subject areas
- Physiology
- Physiology (medical)
- Medicine(all)