Influence of ultrasonic agitation on bond strength, marginal adaptation, and tooth discoloration provided by three coronary barrier endodontic materials

Research output: Contribution to journalArticle


  • Bernardo A Aguiar
  • Luciana M A Frota
  • Daniela T Taguatinga
  • Rodrigo R Vivan
  • Marco A H Duarte
  • Bruno Carvalho de Vasconcelos

Colleges, School and Institutes

External organisations

  • Department of Endodontics, Faculty of Dentistry, University of Fortaleza, Fortaleza, Ceará, Brazil.
  • Post-Graduate Program in Dentistry, School of Pharmacy, Dentistry and Nursing, Federal University of Ceará, Fortaleza, CE, Brazil.
  • Endodontist Private Practice, Goiania, GO, Brazil.
  • Department of Dentistry, Endodontics and Dental Materials, Dental School of Bauru, University of São Paulo - USP, Bauru, SP, Brazil.
  • Periodontal Research Group; Institute of Clinical Sciences; College of Medical and Dental Sciences; School of Dentistry; University of Birmingham; Birmingham Community Health Trust; Birmingham UK
  • School of Dentistry of Sobral, Federal University of Ceará, Campus Sobral, Sobral, CE, Brazil.


Objectives: The effect of ultrasonic agitation (UA) on bond strength and adaptation of cervical plugs prepared with MTA Angelus (MTA), MTA Repair HP (MTAHP), and Biodentine (BIO) was evaluated. Dentin discoloration caused by the materials/treatment was also assessed.

Materials and methods: Seventy-two single rooted teeth were divided into six groups depending on the materials/treatment. After cervical plug preparation, dentin discs were excised for the push-out test; additional discs were analyzed under the confocal microscope to determine adaptation (gaps occurrence). For dentin discoloration analysis (ΔE), blocks of bovine incisors had cavities prepared and filled with the materials/treatment (from 7 to 180 days).

Results: Both bond strength and adaptation were positively influenced by UA (P < 0.05). Comparison between materials showed an advantage for BIO when compared to MTAHP (P < 0.05). The best and worst results were provided by BIO/UA (12.66 MPa and 1.87%) and MTAHP (2.54 MPa and 28.58%), respectively. For ΔE, significant differences were observed throughout the periods. Just the MTA without UA exhibited noticeable discoloration at 180 days (P < 0.05).

Conclusions: UA favored a better adaptation of the materials to the dentin root, resulting in higher bond strength and adaptation of the materials to the root canal walls. Moreover, UA reduced MTA discoloration, keeping it imperceptible over the period evaluated.

Clinical relevance: The better adaptation and higher bond strength provided by UA can be considered clinically relevant due to the importance of maintaining blood clot integrity and the possible esthetic compromise provided by reparative materials when used as coronary barrier in regenerative procedures.


Original languageEnglish
Pages (from-to)4113-4122
Number of pages10
JournalClinical Oral Investigations
Issue number11
Early online date20 Feb 2019
Publication statusPublished - Nov 2019


  • Endodontics, Hydraulic calcium silicate cement, Marginal adaptation, Tooth discoloration, Ultrasonic agitation

ASJC Scopus subject areas