Inflammation and tissue repair markers distinguish the nodular sclerosis and mixed cellularity subtypes of classical Hodgkin's lymphoma

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Inflammation and tissue repair markers distinguish the nodular sclerosis and mixed cellularity subtypes of classical Hodgkin's lymphoma. / Birgersdotter, A; Baumforth, Karl; Porwit, A; Sjöberg, J; Wei, Wenbin; Björkholm, M; Murray, Paul; Ernberg, I.

In: British Journal of Cancer, Vol. 101, No. 8, 20.10.2009, p. 1393-1401.

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@article{01a7e58c3798428db8f0c29cbd94eaaf,
title = "Inflammation and tissue repair markers distinguish the nodular sclerosis and mixed cellularity subtypes of classical Hodgkin's lymphoma",
abstract = "BACKGROUND: Classical Hodgkin's lymphoma (cHL), although a malignant disease, has many features in common with an inflammatory condition. The aim of this study was to establish the molecular characteristics of the two most common cHL subtypes, nodular sclerosis (NS) and mixed cellularity (MC), based on molecular profiling and immunohistochemistry, with special reference to the inflammatory microenvironment. METHODS: We analysed 44 gene expression profiles of cHL whole tumour tissues, 25 cases of NS and 19 cases of MC, using Affymetrix chip technology and immunohistochemistry. RESULTS: In the NS subtype, 152 genes showed a significantly higher expression, including genes involved in extracellular matrix (ECM) remodelling and ECM deposition similar to wound healing. Among these were SPARC, CTSK and COLI. Immunohistochemistry revealed that the NS-related genes were mainly expressed by macrophages and fibroblasts. Fifty-three genes had a higher expression in the MC subtype, including several inflammation-related genes, such as C1Q alpha, C1Q beta and CXCL9. In MC tissues, the C1Q subunits were mainly expressed by infiltrating macrophages. CONCLUSIONS AND INTERPRETATIONS: We suggest that the identified subtype-specific genes could reflect different phases of wound healing. Our study underlines the potential function of infiltrating macrophages in shaping the cHL tumour microenvironment. British Journal of Cancer (2009) 101, 1393-1401. doi:10.1038/sj.bjc.6605238 www.bjcancer.com Published online 22 September 2009 (C) 2009 Cancer Research UK",
keywords = "wound healing, Hodgkin's lymphoma, gene expression, microenvironment",
author = "A Birgersdotter and Karl Baumforth and A Porwit and J Sj{\"o}berg and Wenbin Wei and M Bj{\"o}rkholm and Paul Murray and I Ernberg",
year = "2009",
month = oct,
day = "20",
doi = "10.1038/sj.bjc.6605238",
language = "English",
volume = "101",
pages = "1393--1401",
journal = "British Journal of Cancer",
issn = "0007-0920",
publisher = "Cancer Research UK",
number = "8",

}

RIS

TY - JOUR

T1 - Inflammation and tissue repair markers distinguish the nodular sclerosis and mixed cellularity subtypes of classical Hodgkin's lymphoma

AU - Birgersdotter, A

AU - Baumforth, Karl

AU - Porwit, A

AU - Sjöberg, J

AU - Wei, Wenbin

AU - Björkholm, M

AU - Murray, Paul

AU - Ernberg, I

PY - 2009/10/20

Y1 - 2009/10/20

N2 - BACKGROUND: Classical Hodgkin's lymphoma (cHL), although a malignant disease, has many features in common with an inflammatory condition. The aim of this study was to establish the molecular characteristics of the two most common cHL subtypes, nodular sclerosis (NS) and mixed cellularity (MC), based on molecular profiling and immunohistochemistry, with special reference to the inflammatory microenvironment. METHODS: We analysed 44 gene expression profiles of cHL whole tumour tissues, 25 cases of NS and 19 cases of MC, using Affymetrix chip technology and immunohistochemistry. RESULTS: In the NS subtype, 152 genes showed a significantly higher expression, including genes involved in extracellular matrix (ECM) remodelling and ECM deposition similar to wound healing. Among these were SPARC, CTSK and COLI. Immunohistochemistry revealed that the NS-related genes were mainly expressed by macrophages and fibroblasts. Fifty-three genes had a higher expression in the MC subtype, including several inflammation-related genes, such as C1Q alpha, C1Q beta and CXCL9. In MC tissues, the C1Q subunits were mainly expressed by infiltrating macrophages. CONCLUSIONS AND INTERPRETATIONS: We suggest that the identified subtype-specific genes could reflect different phases of wound healing. Our study underlines the potential function of infiltrating macrophages in shaping the cHL tumour microenvironment. British Journal of Cancer (2009) 101, 1393-1401. doi:10.1038/sj.bjc.6605238 www.bjcancer.com Published online 22 September 2009 (C) 2009 Cancer Research UK

AB - BACKGROUND: Classical Hodgkin's lymphoma (cHL), although a malignant disease, has many features in common with an inflammatory condition. The aim of this study was to establish the molecular characteristics of the two most common cHL subtypes, nodular sclerosis (NS) and mixed cellularity (MC), based on molecular profiling and immunohistochemistry, with special reference to the inflammatory microenvironment. METHODS: We analysed 44 gene expression profiles of cHL whole tumour tissues, 25 cases of NS and 19 cases of MC, using Affymetrix chip technology and immunohistochemistry. RESULTS: In the NS subtype, 152 genes showed a significantly higher expression, including genes involved in extracellular matrix (ECM) remodelling and ECM deposition similar to wound healing. Among these were SPARC, CTSK and COLI. Immunohistochemistry revealed that the NS-related genes were mainly expressed by macrophages and fibroblasts. Fifty-three genes had a higher expression in the MC subtype, including several inflammation-related genes, such as C1Q alpha, C1Q beta and CXCL9. In MC tissues, the C1Q subunits were mainly expressed by infiltrating macrophages. CONCLUSIONS AND INTERPRETATIONS: We suggest that the identified subtype-specific genes could reflect different phases of wound healing. Our study underlines the potential function of infiltrating macrophages in shaping the cHL tumour microenvironment. British Journal of Cancer (2009) 101, 1393-1401. doi:10.1038/sj.bjc.6605238 www.bjcancer.com Published online 22 September 2009 (C) 2009 Cancer Research UK

KW - wound healing

KW - Hodgkin's lymphoma

KW - gene expression

KW - microenvironment

U2 - 10.1038/sj.bjc.6605238

DO - 10.1038/sj.bjc.6605238

M3 - Article

C2 - 19773754

VL - 101

SP - 1393

EP - 1401

JO - British Journal of Cancer

JF - British Journal of Cancer

SN - 0007-0920

IS - 8

ER -