Inertial picobalance reveals fast mass fluctuations in mammalian cells

Research output: Contribution to journalArticlepeer-review

Standard

Inertial picobalance reveals fast mass fluctuations in mammalian cells. / Martínez-Martín, David; Fläschner, Gotthold; Gaub, Benjamin; Martin, Sascha; Newton, Richard; Beerli, Corina; Mercer, Jason; Gerber, Christoph; Müller, Daniel J.

In: Nature, Vol. 550, No. 7677, 25.10.2017, p. 500-505.

Research output: Contribution to journalArticlepeer-review

Harvard

Martínez-Martín, D, Fläschner, G, Gaub, B, Martin, S, Newton, R, Beerli, C, Mercer, J, Gerber, C & Müller, DJ 2017, 'Inertial picobalance reveals fast mass fluctuations in mammalian cells', Nature, vol. 550, no. 7677, pp. 500-505. https://doi.org/10.1038/nature24288

APA

Martínez-Martín, D., Fläschner, G., Gaub, B., Martin, S., Newton, R., Beerli, C., Mercer, J., Gerber, C., & Müller, D. J. (2017). Inertial picobalance reveals fast mass fluctuations in mammalian cells. Nature, 550(7677), 500-505. https://doi.org/10.1038/nature24288

Vancouver

Martínez-Martín D, Fläschner G, Gaub B, Martin S, Newton R, Beerli C et al. Inertial picobalance reveals fast mass fluctuations in mammalian cells. Nature. 2017 Oct 25;550(7677):500-505. https://doi.org/10.1038/nature24288

Author

Martínez-Martín, David ; Fläschner, Gotthold ; Gaub, Benjamin ; Martin, Sascha ; Newton, Richard ; Beerli, Corina ; Mercer, Jason ; Gerber, Christoph ; Müller, Daniel J. / Inertial picobalance reveals fast mass fluctuations in mammalian cells. In: Nature. 2017 ; Vol. 550, No. 7677. pp. 500-505.

Bibtex

@article{d34bb2bf319e4aefa28191c5349abba3,
title = "Inertial picobalance reveals fast mass fluctuations in mammalian cells",
abstract = "The regulation of size, volume and mass in living cells is physiologically important, and dysregulation of these parameters gives rise to many diseases. Cell mass is largely determined by the amount of water, proteins, lipids, carbohydrates and nucleic acids present in a cell, and is tightly linked to metabolism, proliferation and gene expression. Technologies have emerged in recent years that make it possible to track the masses of single suspended cells and adherent cells. However, it has not been possible to track individual adherent cells in physiological conditions at the mass and time resolutions required to observe fast cellular dynamics. Here we introduce a cell balance (a {\^a} € picobalance'), based on an optically excited microresonator, that measures the total mass of single or multiple adherent cells in culture conditions over days with millisecond time resolution and picogram mass sensitivity. Using our technique, we observe that the mass of living mammalian cells fluctuates intrinsically by around one to four per cent over timescales of seconds throughout the cell cycle. Perturbation experiments link these mass fluctuations to the basic cellular processes of ATP synthesis and water transport. Furthermore, we show that growth and cell cycle progression are arrested in cells infected with vaccinia virus, but mass fluctuations continue until cell death. Our measurements suggest that all living cells show fast and subtle mass fluctuations throughout the cell cycle. As our cell balance is easy to handle and compatible with fluorescence microscopy, we anticipate that our approach will contribute to the understanding of cell mass regulation in various cell states and across timescales, which is important in areas including physiology, cancer research, stem-cell differentiation and drug discovery.",
author = "David Mart{\'i}nez-Mart{\'i}n and Gotthold Fl{\"a}schner and Benjamin Gaub and Sascha Martin and Richard Newton and Corina Beerli and Jason Mercer and Christoph Gerber and M{\"u}ller, {Daniel J.}",
year = "2017",
month = oct,
day = "25",
doi = "10.1038/nature24288",
language = "English",
volume = "550",
pages = "500--505",
journal = "Nature",
issn = "0028-0836",
publisher = "Nature Publishing Group",
number = "7677",

}

RIS

TY - JOUR

T1 - Inertial picobalance reveals fast mass fluctuations in mammalian cells

AU - Martínez-Martín, David

AU - Fläschner, Gotthold

AU - Gaub, Benjamin

AU - Martin, Sascha

AU - Newton, Richard

AU - Beerli, Corina

AU - Mercer, Jason

AU - Gerber, Christoph

AU - Müller, Daniel J.

PY - 2017/10/25

Y1 - 2017/10/25

N2 - The regulation of size, volume and mass in living cells is physiologically important, and dysregulation of these parameters gives rise to many diseases. Cell mass is largely determined by the amount of water, proteins, lipids, carbohydrates and nucleic acids present in a cell, and is tightly linked to metabolism, proliferation and gene expression. Technologies have emerged in recent years that make it possible to track the masses of single suspended cells and adherent cells. However, it has not been possible to track individual adherent cells in physiological conditions at the mass and time resolutions required to observe fast cellular dynamics. Here we introduce a cell balance (a â € picobalance'), based on an optically excited microresonator, that measures the total mass of single or multiple adherent cells in culture conditions over days with millisecond time resolution and picogram mass sensitivity. Using our technique, we observe that the mass of living mammalian cells fluctuates intrinsically by around one to four per cent over timescales of seconds throughout the cell cycle. Perturbation experiments link these mass fluctuations to the basic cellular processes of ATP synthesis and water transport. Furthermore, we show that growth and cell cycle progression are arrested in cells infected with vaccinia virus, but mass fluctuations continue until cell death. Our measurements suggest that all living cells show fast and subtle mass fluctuations throughout the cell cycle. As our cell balance is easy to handle and compatible with fluorescence microscopy, we anticipate that our approach will contribute to the understanding of cell mass regulation in various cell states and across timescales, which is important in areas including physiology, cancer research, stem-cell differentiation and drug discovery.

AB - The regulation of size, volume and mass in living cells is physiologically important, and dysregulation of these parameters gives rise to many diseases. Cell mass is largely determined by the amount of water, proteins, lipids, carbohydrates and nucleic acids present in a cell, and is tightly linked to metabolism, proliferation and gene expression. Technologies have emerged in recent years that make it possible to track the masses of single suspended cells and adherent cells. However, it has not been possible to track individual adherent cells in physiological conditions at the mass and time resolutions required to observe fast cellular dynamics. Here we introduce a cell balance (a â € picobalance'), based on an optically excited microresonator, that measures the total mass of single or multiple adherent cells in culture conditions over days with millisecond time resolution and picogram mass sensitivity. Using our technique, we observe that the mass of living mammalian cells fluctuates intrinsically by around one to four per cent over timescales of seconds throughout the cell cycle. Perturbation experiments link these mass fluctuations to the basic cellular processes of ATP synthesis and water transport. Furthermore, we show that growth and cell cycle progression are arrested in cells infected with vaccinia virus, but mass fluctuations continue until cell death. Our measurements suggest that all living cells show fast and subtle mass fluctuations throughout the cell cycle. As our cell balance is easy to handle and compatible with fluorescence microscopy, we anticipate that our approach will contribute to the understanding of cell mass regulation in various cell states and across timescales, which is important in areas including physiology, cancer research, stem-cell differentiation and drug discovery.

UR - http://www.scopus.com/inward/record.url?scp=85032456698&partnerID=8YFLogxK

U2 - 10.1038/nature24288

DO - 10.1038/nature24288

M3 - Article

C2 - 29072271

AN - SCOPUS:85032456698

VL - 550

SP - 500

EP - 505

JO - Nature

JF - Nature

SN - 0028-0836

IS - 7677

ER -