Increasing carbohydrate oxidation improves contractile reserves and prevents hypertrophy in porcine right heart failure

Research output: Contribution to journalArticlepeer-review

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Increasing carbohydrate oxidation improves contractile reserves and prevents hypertrophy in porcine right heart failure. / Bøgh, Nikolaj; Hansen, Esben S S; Omann, Camilla; Lindhardt, Jakob; Nielsen, Per M; Stephenson, Robert S; Laustsen, Christoffer; Hjortdal, Vibeke E; Agger, Peter.

In: Scientific Reports, Vol. 10, No. 1, 18.05.2020, p. 8158.

Research output: Contribution to journalArticlepeer-review

Harvard

Bøgh, N, Hansen, ESS, Omann, C, Lindhardt, J, Nielsen, PM, Stephenson, RS, Laustsen, C, Hjortdal, VE & Agger, P 2020, 'Increasing carbohydrate oxidation improves contractile reserves and prevents hypertrophy in porcine right heart failure', Scientific Reports, vol. 10, no. 1, pp. 8158. https://doi.org/10.1038/s41598-020-65098-7

APA

Bøgh, N., Hansen, E. S. S., Omann, C., Lindhardt, J., Nielsen, P. M., Stephenson, R. S., Laustsen, C., Hjortdal, V. E., & Agger, P. (2020). Increasing carbohydrate oxidation improves contractile reserves and prevents hypertrophy in porcine right heart failure. Scientific Reports, 10(1), 8158. https://doi.org/10.1038/s41598-020-65098-7

Vancouver

Author

Bøgh, Nikolaj ; Hansen, Esben S S ; Omann, Camilla ; Lindhardt, Jakob ; Nielsen, Per M ; Stephenson, Robert S ; Laustsen, Christoffer ; Hjortdal, Vibeke E ; Agger, Peter. / Increasing carbohydrate oxidation improves contractile reserves and prevents hypertrophy in porcine right heart failure. In: Scientific Reports. 2020 ; Vol. 10, No. 1. pp. 8158.

Bibtex

@article{8339f27bfb2042338dc94479fbde95ad,
title = "Increasing carbohydrate oxidation improves contractile reserves and prevents hypertrophy in porcine right heart failure",
abstract = "In heart failure, myocardial overload causes vast metabolic changes that impair cardiac energy production and contribute to deterioration of contractile function. However, metabolic therapy is not used in heart failure care. We aimed to investigate the interplay between cardiac function and myocardial carbohydrate metabolism in a large animal heart failure model. Using magnetic resonance spectroscopy with hyperpolarized pyruvate and magnetic resonance imaging at rest and during pharmacological stress, we investigated the in-vivo cardiac pyruvate metabolism and contractility in a porcine model of chronic pulmonary insufficiency causing right ventricular volume overload. To assess if increasing the carbohydrate metabolic reserve improves the contractile reserve, a group of animals were fed dichloroacetate, an activator of pyruvate oxidation. Volume overload caused heart failure with decreased pyruvate dehydrogenase flux and poor ejection fraction reserve. The animals treated with dichloroacetate had a larger contractile response to dobutamine stress than non-treated animals. Further, dichloroacetate prevented myocardial hypertrophy. The in-vivo metabolic data were validated by mitochondrial respirometry, enzyme activity assays and gene expression analyses. Our results show that pyruvate dehydrogenase kinase inhibition improves the contractile reserve and decreases hypertrophy by augmenting carbohydrate metabolism in porcine heart failure. The approach is promising for metabolic heart failure therapy.",
author = "Nikolaj B{\o}gh and Hansen, {Esben S S} and Camilla Omann and Jakob Lindhardt and Nielsen, {Per M} and Stephenson, {Robert S} and Christoffer Laustsen and Hjortdal, {Vibeke E} and Peter Agger",
year = "2020",
month = may,
day = "18",
doi = "10.1038/s41598-020-65098-7",
language = "English",
volume = "10",
pages = "8158",
journal = "Scientific Reports",
issn = "2045-2322",
publisher = "Nature Publishing Group",
number = "1",

}

RIS

TY - JOUR

T1 - Increasing carbohydrate oxidation improves contractile reserves and prevents hypertrophy in porcine right heart failure

AU - Bøgh, Nikolaj

AU - Hansen, Esben S S

AU - Omann, Camilla

AU - Lindhardt, Jakob

AU - Nielsen, Per M

AU - Stephenson, Robert S

AU - Laustsen, Christoffer

AU - Hjortdal, Vibeke E

AU - Agger, Peter

PY - 2020/5/18

Y1 - 2020/5/18

N2 - In heart failure, myocardial overload causes vast metabolic changes that impair cardiac energy production and contribute to deterioration of contractile function. However, metabolic therapy is not used in heart failure care. We aimed to investigate the interplay between cardiac function and myocardial carbohydrate metabolism in a large animal heart failure model. Using magnetic resonance spectroscopy with hyperpolarized pyruvate and magnetic resonance imaging at rest and during pharmacological stress, we investigated the in-vivo cardiac pyruvate metabolism and contractility in a porcine model of chronic pulmonary insufficiency causing right ventricular volume overload. To assess if increasing the carbohydrate metabolic reserve improves the contractile reserve, a group of animals were fed dichloroacetate, an activator of pyruvate oxidation. Volume overload caused heart failure with decreased pyruvate dehydrogenase flux and poor ejection fraction reserve. The animals treated with dichloroacetate had a larger contractile response to dobutamine stress than non-treated animals. Further, dichloroacetate prevented myocardial hypertrophy. The in-vivo metabolic data were validated by mitochondrial respirometry, enzyme activity assays and gene expression analyses. Our results show that pyruvate dehydrogenase kinase inhibition improves the contractile reserve and decreases hypertrophy by augmenting carbohydrate metabolism in porcine heart failure. The approach is promising for metabolic heart failure therapy.

AB - In heart failure, myocardial overload causes vast metabolic changes that impair cardiac energy production and contribute to deterioration of contractile function. However, metabolic therapy is not used in heart failure care. We aimed to investigate the interplay between cardiac function and myocardial carbohydrate metabolism in a large animal heart failure model. Using magnetic resonance spectroscopy with hyperpolarized pyruvate and magnetic resonance imaging at rest and during pharmacological stress, we investigated the in-vivo cardiac pyruvate metabolism and contractility in a porcine model of chronic pulmonary insufficiency causing right ventricular volume overload. To assess if increasing the carbohydrate metabolic reserve improves the contractile reserve, a group of animals were fed dichloroacetate, an activator of pyruvate oxidation. Volume overload caused heart failure with decreased pyruvate dehydrogenase flux and poor ejection fraction reserve. The animals treated with dichloroacetate had a larger contractile response to dobutamine stress than non-treated animals. Further, dichloroacetate prevented myocardial hypertrophy. The in-vivo metabolic data were validated by mitochondrial respirometry, enzyme activity assays and gene expression analyses. Our results show that pyruvate dehydrogenase kinase inhibition improves the contractile reserve and decreases hypertrophy by augmenting carbohydrate metabolism in porcine heart failure. The approach is promising for metabolic heart failure therapy.

U2 - 10.1038/s41598-020-65098-7

DO - 10.1038/s41598-020-65098-7

M3 - Article

C2 - 32424129

VL - 10

SP - 8158

JO - Scientific Reports

JF - Scientific Reports

SN - 2045-2322

IS - 1

ER -