In vivo EPR spectroscopy: biomedical and potential diagnostic applications

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In vivo EPR spectroscopy : biomedical and potential diagnostic applications. / Jackson, Simon K; Thomas, Matthew P; Smith, Sam; Madhani, Melanie; Rogers, Stephen C; James, Philip E.

In: Faraday Discussions, Vol. 126, 2004, p. 103-17; discussion 169-83.

Research output: Contribution to journalArticlepeer-review

Harvard

Jackson, SK, Thomas, MP, Smith, S, Madhani, M, Rogers, SC & James, PE 2004, 'In vivo EPR spectroscopy: biomedical and potential diagnostic applications', Faraday Discussions, vol. 126, pp. 103-17; discussion 169-83. https://doi.org/10.1039/B307162F

APA

Jackson, S. K., Thomas, M. P., Smith, S., Madhani, M., Rogers, S. C., & James, P. E. (2004). In vivo EPR spectroscopy: biomedical and potential diagnostic applications. Faraday Discussions, 126, 103-17; discussion 169-83. https://doi.org/10.1039/B307162F

Vancouver

Jackson SK, Thomas MP, Smith S, Madhani M, Rogers SC, James PE. In vivo EPR spectroscopy: biomedical and potential diagnostic applications. Faraday Discussions. 2004;126:103-17; discussion 169-83. https://doi.org/10.1039/B307162F

Author

Jackson, Simon K ; Thomas, Matthew P ; Smith, Sam ; Madhani, Melanie ; Rogers, Stephen C ; James, Philip E. / In vivo EPR spectroscopy : biomedical and potential diagnostic applications. In: Faraday Discussions. 2004 ; Vol. 126. pp. 103-17; discussion 169-83.

Bibtex

@article{c778d612858840bfb7dc1f80f0523d8f,
title = "In vivo EPR spectroscopy: biomedical and potential diagnostic applications",
abstract = "EPR spectroscopic techniques have been developed for the measurement of oxygen and nitric oxide in vivo. Specifically, the methods for in vivo measurement of these molecules has been applied to the study of septic shock, utilising an experimental murine model developed in our laboratory. Oxygen was measured as pO2 by the particlulate probes Gloxy and LiPc, which were surgically implanted at specific sites in tissues, and the soluble probe Trityl, which was administered intravenously. Nitric oxide was measured as the NO-Fe-(DETC)2 complex after administration of Fe2+ and DETC. LPS was seen to significantly decrease liver oxygen measured across the lobule and at the sinusoids by the Gloxy probe; there was a corresponding increase in nitric oxide both in the liver and systemically. The nitric oxide most likely originated from increased iNOS enzyme in the liver as demonstrated by Western blotting and the localisation of nitric oxide to the liver was confirmed with EPR imaging. LPS also caused a decrease in cerebral blood and tissue oxygenation, the rate of which was found to be dependent on the blood oxygenation. The development and applications of these in vivo EPR techniques for biomedical research and diagnostics is discussed.",
keywords = "Animals, Brain Chemistry, Diagnostic Imaging, Electron Spin Resonance Spectroscopy, Free Radicals, Lipopolysaccharides, Liver, Mice, Nitric Oxide, Oxygen Consumption, Rats, Shock, Septic",
author = "Jackson, {Simon K} and Thomas, {Matthew P} and Sam Smith and Melanie Madhani and Rogers, {Stephen C} and James, {Philip E}",
year = "2004",
doi = "10.1039/B307162F",
language = "English",
volume = "126",
pages = "103--17; discussion 169--83",
journal = "Faraday Discussions",
issn = "1359-6640",
publisher = "Royal Society of Chemistry",

}

RIS

TY - JOUR

T1 - In vivo EPR spectroscopy

T2 - biomedical and potential diagnostic applications

AU - Jackson, Simon K

AU - Thomas, Matthew P

AU - Smith, Sam

AU - Madhani, Melanie

AU - Rogers, Stephen C

AU - James, Philip E

PY - 2004

Y1 - 2004

N2 - EPR spectroscopic techniques have been developed for the measurement of oxygen and nitric oxide in vivo. Specifically, the methods for in vivo measurement of these molecules has been applied to the study of septic shock, utilising an experimental murine model developed in our laboratory. Oxygen was measured as pO2 by the particlulate probes Gloxy and LiPc, which were surgically implanted at specific sites in tissues, and the soluble probe Trityl, which was administered intravenously. Nitric oxide was measured as the NO-Fe-(DETC)2 complex after administration of Fe2+ and DETC. LPS was seen to significantly decrease liver oxygen measured across the lobule and at the sinusoids by the Gloxy probe; there was a corresponding increase in nitric oxide both in the liver and systemically. The nitric oxide most likely originated from increased iNOS enzyme in the liver as demonstrated by Western blotting and the localisation of nitric oxide to the liver was confirmed with EPR imaging. LPS also caused a decrease in cerebral blood and tissue oxygenation, the rate of which was found to be dependent on the blood oxygenation. The development and applications of these in vivo EPR techniques for biomedical research and diagnostics is discussed.

AB - EPR spectroscopic techniques have been developed for the measurement of oxygen and nitric oxide in vivo. Specifically, the methods for in vivo measurement of these molecules has been applied to the study of septic shock, utilising an experimental murine model developed in our laboratory. Oxygen was measured as pO2 by the particlulate probes Gloxy and LiPc, which were surgically implanted at specific sites in tissues, and the soluble probe Trityl, which was administered intravenously. Nitric oxide was measured as the NO-Fe-(DETC)2 complex after administration of Fe2+ and DETC. LPS was seen to significantly decrease liver oxygen measured across the lobule and at the sinusoids by the Gloxy probe; there was a corresponding increase in nitric oxide both in the liver and systemically. The nitric oxide most likely originated from increased iNOS enzyme in the liver as demonstrated by Western blotting and the localisation of nitric oxide to the liver was confirmed with EPR imaging. LPS also caused a decrease in cerebral blood and tissue oxygenation, the rate of which was found to be dependent on the blood oxygenation. The development and applications of these in vivo EPR techniques for biomedical research and diagnostics is discussed.

KW - Animals

KW - Brain Chemistry

KW - Diagnostic Imaging

KW - Electron Spin Resonance Spectroscopy

KW - Free Radicals

KW - Lipopolysaccharides

KW - Liver

KW - Mice

KW - Nitric Oxide

KW - Oxygen Consumption

KW - Rats

KW - Shock, Septic

U2 - 10.1039/B307162F

DO - 10.1039/B307162F

M3 - Article

C2 - 14992402

VL - 126

SP - 103-17; discussion 169-83

JO - Faraday Discussions

JF - Faraday Discussions

SN - 1359-6640

ER -