In vitro migration assays

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In vitro migration assays. / Taylor, Lewis; Recio, Carlota; Greaves, David R.; Iqbal, Asif Jilani.

In: Methods in molecular biology, Vol. 1784, 15.05.2018, p. 197-214.

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Taylor, Lewis ; Recio, Carlota ; Greaves, David R. ; Iqbal, Asif Jilani. / In vitro migration assays. In: Methods in molecular biology. 2018 ; Vol. 1784. pp. 197-214.

Bibtex

@article{0fdeab8b43044062a713206092e49fe4,
title = "In vitro migration assays",
abstract = "The timely recruitment of innate and adaptive immune cells to sites of inflammation and repair is essential for host defense against pathogens and repair of damaged tissues. The development of bioassays such as in vitro chemotaxis assays played an important role in the original purification of chemoattractant cytokines including interleukin-1 and the CC and CXC chemokines. The earliest chemotaxis methods were based on the principle of the Boyden chamber, first described in 1962. In this chapter we give detailed protocols for more recent techniques that allow determination of macrophage chemotaxis in real time. These techniques have given new insights into the regulation of macrophage responses to chemotaxis in vitro and in vivo.",
keywords = "Chemotaxis, Macrophages, Inflammation, Chemokines, Migration",
author = "Lewis Taylor and Carlota Recio and Greaves, {David R.} and Iqbal, {Asif Jilani}",
year = "2018",
month = may,
day = "15",
doi = "10.1007/978-1-4939-7837-3_19",
language = "English",
volume = "1784",
pages = "197--214",
journal = "Methods in molecular biology",
issn = "1064-3745",
publisher = "Springer",

}

RIS

TY - JOUR

T1 - In vitro migration assays

AU - Taylor, Lewis

AU - Recio, Carlota

AU - Greaves, David R.

AU - Iqbal, Asif Jilani

PY - 2018/5/15

Y1 - 2018/5/15

N2 - The timely recruitment of innate and adaptive immune cells to sites of inflammation and repair is essential for host defense against pathogens and repair of damaged tissues. The development of bioassays such as in vitro chemotaxis assays played an important role in the original purification of chemoattractant cytokines including interleukin-1 and the CC and CXC chemokines. The earliest chemotaxis methods were based on the principle of the Boyden chamber, first described in 1962. In this chapter we give detailed protocols for more recent techniques that allow determination of macrophage chemotaxis in real time. These techniques have given new insights into the regulation of macrophage responses to chemotaxis in vitro and in vivo.

AB - The timely recruitment of innate and adaptive immune cells to sites of inflammation and repair is essential for host defense against pathogens and repair of damaged tissues. The development of bioassays such as in vitro chemotaxis assays played an important role in the original purification of chemoattractant cytokines including interleukin-1 and the CC and CXC chemokines. The earliest chemotaxis methods were based on the principle of the Boyden chamber, first described in 1962. In this chapter we give detailed protocols for more recent techniques that allow determination of macrophage chemotaxis in real time. These techniques have given new insights into the regulation of macrophage responses to chemotaxis in vitro and in vivo.

KW - Chemotaxis

KW - Macrophages

KW - Inflammation

KW - Chemokines

KW - Migration

U2 - 10.1007/978-1-4939-7837-3_19

DO - 10.1007/978-1-4939-7837-3_19

M3 - Article

C2 - 29761401

VL - 1784

SP - 197

EP - 214

JO - Methods in molecular biology

JF - Methods in molecular biology

SN - 1064-3745

ER -