In Vitro and Ex Vivo Models to Study T Cell Migration Through the Human Liver Parenchyma

Benjamin G Wiggins; Konstantinos Aliazis; Scott P Davies; Gideon Hirschfield; Patricia F Lalor; Gary Reynolds; Zania Stamataki

doi:10.1007/978-1-4939-6931-9_14

In Vitro and Ex Vivo Models to Study T Cell Migration Through the Human Liver Parenchyma

Benjamin G Wiggins, Konstantinos Aliazis, Scott P Davies, Gideon Hirschfield, Patricia F Lalor, Gary Reynolds, Zania Stamataki

Immunology and Immunotherapy

Research output: Chapter in Book/Report/Conference proceeding › Chapter (peer-reviewed) › peer-review

Abstract

The liver is the largest internal organ and filters around 3 pints of blood per minute. This continuous flux of blood should not be confused with rapid egress of lymphocytes through the liver; this organ has intricate corridors of specialized sinusoidal spaces, ensuring that immune cells decelerate to shear flow rates, and providing ample opportunities to interact with parenchymal cells. Migration has been intricately linked to T cell function; it is therefore important to study liver T cell biology into context within the liver microenvironment. Here we discuss the highly organized architecture of liver-resident cells (sinusoidal endothelia, Kupffer cells, stellate cells/myofibroblasts, and biliary and hepatic epithelia) and showcase basic, multicellular, and complex systems to model T cell migration through the human liver microenvironment in vitro and ex vivo.

Original language	English
Title of host publication	T-Cell Trafficking:
Subtitle of host publication	Methods and Protocols
Editors	George Edward Rainger, Helen M. Mcgettrick
Publisher	Springer
Pages	195-214
Number of pages	20
Volume	1591
ISBN (Electronic)	9781493969319
ISBN (Print)	9781493969296
DOIs	https://doi.org/10.1007/978-1-4939-6931-9_14
Publication status	Published - 28 Mar 2017

Publication series

Name	Methods in Molecular Biology
Publisher	Springer
ISSN (Print)	1064-3745

Keywords

Journal Article
adhesion
cell culture
coculture
cytokines
inflammation
liver
liver wedge
lymphocytes
migration
perfusion
peristaltic pump
T cells

Access to Document

10.1007/978-1-4939-6931-9_14Licence: None: All rights reserved

https://link.springer.com/protocol/10.1007%2F978-1-4939-6931-9_14Licence: None: All rights reserved

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Wiggins, B. G., Aliazis, K., Davies, S. P., Hirschfield, G., Lalor, P. F., Reynolds, G., & Stamataki, Z. (2017). In Vitro and Ex Vivo Models to Study T Cell Migration Through the Human Liver Parenchyma. In G. E. Rainger, & H. M. Mcgettrick (Eds.), T-Cell Trafficking: Methods and Protocols (Vol. 1591, pp. 195-214). (Methods in Molecular Biology). Springer. https://doi.org/10.1007/978-1-4939-6931-9_14

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abstract = "The liver is the largest internal organ and filters around 3 pints of blood per minute. This continuous flux of blood should not be confused with rapid egress of lymphocytes through the liver; this organ has intricate corridors of specialized sinusoidal spaces, ensuring that immune cells decelerate to shear flow rates, and providing ample opportunities to interact with parenchymal cells. Migration has been intricately linked to T cell function; it is therefore important to study liver T cell biology into context within the liver microenvironment. Here we discuss the highly organized architecture of liver-resident cells (sinusoidal endothelia, Kupffer cells, stellate cells/myofibroblasts, and biliary and hepatic epithelia) and showcase basic, multicellular, and complex systems to model T cell migration through the human liver microenvironment in vitro and ex vivo.",

keywords = "Journal Article, adhesion, cell culture , coculture , cytokines , inflammation , liver , liver wedge , lymphocytes , migration , perfusion , peristaltic pump , T cells",

author = "Wiggins, {Benjamin G} and Konstantinos Aliazis and Davies, {Scott P} and Gideon Hirschfield and Lalor, {Patricia F} and Gary Reynolds and Zania Stamataki",

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language = "English",

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Wiggins, BG, Aliazis, K, Davies, SP, Hirschfield, G, Lalor, PF , Reynolds, G & Stamataki, Z 2017, In Vitro and Ex Vivo Models to Study T Cell Migration Through the Human Liver Parenchyma. in GE Rainger & HM Mcgettrick (eds), T-Cell Trafficking: Methods and Protocols. vol. 1591, Methods in Molecular Biology, Springer, pp. 195-214. https://doi.org/10.1007/978-1-4939-6931-9_14

In Vitro and Ex Vivo Models to Study T Cell Migration Through the Human Liver Parenchyma. / Wiggins, Benjamin G; Aliazis, Konstantinos; Davies, Scott P et al.
T-Cell Trafficking: Methods and Protocols. ed. / George Edward Rainger; Helen M. Mcgettrick. Vol. 1591 Springer, 2017. p. 195-214 (Methods in Molecular Biology).

Research output: Chapter in Book/Report/Conference proceeding › Chapter (peer-reviewed) › peer-review

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T1 - In Vitro and Ex Vivo Models to Study T Cell Migration Through the Human Liver Parenchyma

AU - Wiggins, Benjamin G

AU - Aliazis, Konstantinos

AU - Davies, Scott P

AU - Hirschfield, Gideon

AU - Lalor, Patricia F

AU - Reynolds, Gary

AU - Stamataki, Zania

PY - 2017/3/28

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N2 - The liver is the largest internal organ and filters around 3 pints of blood per minute. This continuous flux of blood should not be confused with rapid egress of lymphocytes through the liver; this organ has intricate corridors of specialized sinusoidal spaces, ensuring that immune cells decelerate to shear flow rates, and providing ample opportunities to interact with parenchymal cells. Migration has been intricately linked to T cell function; it is therefore important to study liver T cell biology into context within the liver microenvironment. Here we discuss the highly organized architecture of liver-resident cells (sinusoidal endothelia, Kupffer cells, stellate cells/myofibroblasts, and biliary and hepatic epithelia) and showcase basic, multicellular, and complex systems to model T cell migration through the human liver microenvironment in vitro and ex vivo.

AB - The liver is the largest internal organ and filters around 3 pints of blood per minute. This continuous flux of blood should not be confused with rapid egress of lymphocytes through the liver; this organ has intricate corridors of specialized sinusoidal spaces, ensuring that immune cells decelerate to shear flow rates, and providing ample opportunities to interact with parenchymal cells. Migration has been intricately linked to T cell function; it is therefore important to study liver T cell biology into context within the liver microenvironment. Here we discuss the highly organized architecture of liver-resident cells (sinusoidal endothelia, Kupffer cells, stellate cells/myofibroblasts, and biliary and hepatic epithelia) and showcase basic, multicellular, and complex systems to model T cell migration through the human liver microenvironment in vitro and ex vivo.

KW - Journal Article

KW - adhesion

KW - cell culture

KW - coculture

KW - cytokines

KW - inflammation

KW - liver

KW - liver wedge

KW - lymphocytes

KW - migration

KW - perfusion

KW - peristaltic pump

KW - T cells

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PB - Springer

ER -

In Vitro and Ex Vivo Models to Study T Cell Migration Through the Human Liver Parenchyma

Abstract

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Keywords

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