In situ-forming robust chitosan-poly(ethylene glycol) hydrogels prepared by copper-free azide-alkyne click reaction for tissue engineering

Research output: Contribution to journalArticlepeer-review


  • V.X. Truong
  • M.P. Ablett
  • H.T.J. Gilbert
  • S.M. Richardson
  • J.A. Hoyland

Colleges, School and Institutes


A water-soluble azide-functionalised chitosan was crosslinked with propiolic acid ester-functional poly(ethylene glycol) using copper-free click chemistry. The resultant hydrogel materials were formed within 5–60 min at 37 °C and resulted in mechanically robust materials with tuneable properties such as swelling, mechanical strength and degradation. Importantly, the hydrogels supported mesenchymal stem cell attachment and proliferation and were also non-toxic to encapsulated cells. As such these studies indicate that the hydrogels have potential to be used as injectable biomaterials for tissue engineering.


Original languageEnglish
Pages (from-to)167-175
JournalBiomaterials Science
Publication statusPublished - 4 Oct 2013


  • copper-free click chemistry, hydrogel, chitosan