Improved sectioning in a slit scanning confocal microscope
Research output: Contribution to journal › Article › peer-review
Colleges, School and Institutes
We describe a simple implementation of a slit scanning confocal microscope to obtain an axial resolution better than that of a point-scanning confocal microscope. Under slit illumination, images of a fluorescent object are captured using an array detector instead of a line detector so that out-of-focus light is recorded and then subtracted from the adjacent images. Axial resolution after background subtraction is 2.2 times better than the slit confocal resolution, and out-of-focus image suppression is calculated to attenuate with defocus faster by 1 order of magnitude than in the point confocal case. (C) 2008 Optical Society of America.
|Number of pages||3|
|Publication status||Published - 15 Aug 2008|