Impact of Ag2S NPs on soil bacterial community – A terrestrial mesocosm approach

Research output: Contribution to journalArticlepeer-review

Standard

Impact of Ag2S NPs on soil bacterial community – A terrestrial mesocosm approach. / Peixoto, S.; Khodaparast, Z.; Cornelis, G.; Lahive, E.; Green Etxabe, A.; Baccaro, M.; Papadiamantis, A. G.; Gonçalves, S. F.; Lynch, I.; Busquets-Fite, M.; Puntes, V.; Loureiro, S.; Henriques, I.

In: Ecotoxicology and Environmental Safety, Vol. 206, 111405, 15.12.2020.

Research output: Contribution to journalArticlepeer-review

Harvard

Peixoto, S, Khodaparast, Z, Cornelis, G, Lahive, E, Green Etxabe, A, Baccaro, M, Papadiamantis, AG, Gonçalves, SF, Lynch, I, Busquets-Fite, M, Puntes, V, Loureiro, S & Henriques, I 2020, 'Impact of Ag2S NPs on soil bacterial community – A terrestrial mesocosm approach', Ecotoxicology and Environmental Safety, vol. 206, 111405. https://doi.org/10.1016/j.ecoenv.2020.111405

APA

Peixoto, S., Khodaparast, Z., Cornelis, G., Lahive, E., Green Etxabe, A., Baccaro, M., Papadiamantis, A. G., Gonçalves, S. F., Lynch, I., Busquets-Fite, M., Puntes, V., Loureiro, S., & Henriques, I. (2020). Impact of Ag2S NPs on soil bacterial community – A terrestrial mesocosm approach. Ecotoxicology and Environmental Safety, 206, [111405]. https://doi.org/10.1016/j.ecoenv.2020.111405

Vancouver

Peixoto S, Khodaparast Z, Cornelis G, Lahive E, Green Etxabe A, Baccaro M et al. Impact of Ag2S NPs on soil bacterial community – A terrestrial mesocosm approach. Ecotoxicology and Environmental Safety. 2020 Dec 15;206. 111405. https://doi.org/10.1016/j.ecoenv.2020.111405

Author

Peixoto, S. ; Khodaparast, Z. ; Cornelis, G. ; Lahive, E. ; Green Etxabe, A. ; Baccaro, M. ; Papadiamantis, A. G. ; Gonçalves, S. F. ; Lynch, I. ; Busquets-Fite, M. ; Puntes, V. ; Loureiro, S. ; Henriques, I. / Impact of Ag2S NPs on soil bacterial community – A terrestrial mesocosm approach. In: Ecotoxicology and Environmental Safety. 2020 ; Vol. 206.

Bibtex

@article{b739a04cc3254e85bb0039912a6b1e9f,
title = "Impact of Ag2S NPs on soil bacterial community – A terrestrial mesocosm approach",
abstract = "Soils might be a final sink for Ag2S nanoparticles (NPs). Still, there are limited data on their effects on soil bacterial communities (SBC). To bridge this gap, we investigated the effects of Ag2S NPs (10 mg kg−1 soil) on the structure and function of SBC in a terrestrial indoor mesocosm, using a multi-species design. During 28 days of exposure, the SBC function-related parameters were analysed in terms of enzymatic activity, community level physiological profile, culture of functional bacterial groups [phosphorous-solubilizing bacteria (P-SB) and heterotrophic bacteria (HB)], and SBC structure was analysed by 16S rRNA gene-targeted denaturing gradient gel electrophoresis. The SBC exposed to Ag2S NPs showed a significative decrease of functional parameters, such as β-glucosidase activity and L-arginine consumption, and increase of the acid phosphatase activity. At the structural level, significantly lower richness and diversity were detected, but at later exposure times compared to the AgNO3 treatment, likely because of a low dissolution rate of Ag2S NPs. In fact, stronger effects were observed in soils spiked with AgNO3, in both functional and structural parameters. Changes in SBC structure seem to negatively correlate with parameters related to phosphorous (acid phosphatase activity) and carbon cycling (abundance of HB, P-SB, and β-glucosidase activity). Our results indicate a significant effect of Ag2S NPs on SBC, specifically on parameters related to carbon and phosphorous cycling, at doses as low as 10 mg kg-1 soil. These effects were only observed after 28 days, highlighting the importance of long-term exposure experiments for slowly dissolving NPs.",
keywords = "Community level physiological profile, Denaturing gradient gel electrophoresis, Indoor Mesocosm, Silver sulfide nanoparticle, Soil bacterial community, Soil enzymatic activity",
author = "S. Peixoto and Z. Khodaparast and G. Cornelis and E. Lahive and {Green Etxabe}, A. and M. Baccaro and Papadiamantis, {A. G.} and Gon{\c c}alves, {S. F.} and I. Lynch and M. Busquets-Fite and V. Puntes and S. Loureiro and I. Henriques",
note = "Funding Information: All the authors were funded by the European Union Horizon 2020 project Nano-FASE (Nanomaterial Fate and Speciation in the Environment; grant no. 646002). SL, IH, SP and ZK received additional financial support from Funda??o para a Ci?ncia e a Tecnologia/Minist?rio da Ci?ncia, Tecnologia e Ensino Superior (FCT/MCTES), through national funds, to CESAM (UIDP/50017/2020 + UIDB/50017/2020). Also, this work was supported by FCT through a PhD grant to Sara Peixoto (SFRH/BD/117738/2016). Funding Information: All the authors were funded by the European Union Horizon 2020 project Nano-FASE ( Nanomaterial Fate and Speciation in the Environment ; grant no. 646002 ). SL, IH, SP and ZK received additional financial support from Funda{\c c}{\~a}o para a Ci{\^e}ncia e a Tecnologia/Minist{\'e}rio da Ci{\^e}ncia, Tecnologia e Ensino Superior ( FCT/MCTES ), through national funds, to CESAM ( UIDP/50017/2020 + UIDB/50017/2020 ). Also, this work was supported by FCT through a PhD grant to Sara Peixoto ( SFRH/BD/117738/2016 ). Publisher Copyright: {\textcopyright} 2020 Elsevier Inc.",
year = "2020",
month = dec,
day = "15",
doi = "10.1016/j.ecoenv.2020.111405",
language = "English",
volume = "206",
journal = "Ecotoxicology and Environmental Safety",
issn = "0147-6513",
publisher = "Elsevier",

}

RIS

TY - JOUR

T1 - Impact of Ag2S NPs on soil bacterial community – A terrestrial mesocosm approach

AU - Peixoto, S.

AU - Khodaparast, Z.

AU - Cornelis, G.

AU - Lahive, E.

AU - Green Etxabe, A.

AU - Baccaro, M.

AU - Papadiamantis, A. G.

AU - Gonçalves, S. F.

AU - Lynch, I.

AU - Busquets-Fite, M.

AU - Puntes, V.

AU - Loureiro, S.

AU - Henriques, I.

N1 - Funding Information: All the authors were funded by the European Union Horizon 2020 project Nano-FASE (Nanomaterial Fate and Speciation in the Environment; grant no. 646002). SL, IH, SP and ZK received additional financial support from Funda??o para a Ci?ncia e a Tecnologia/Minist?rio da Ci?ncia, Tecnologia e Ensino Superior (FCT/MCTES), through national funds, to CESAM (UIDP/50017/2020 + UIDB/50017/2020). Also, this work was supported by FCT through a PhD grant to Sara Peixoto (SFRH/BD/117738/2016). Funding Information: All the authors were funded by the European Union Horizon 2020 project Nano-FASE ( Nanomaterial Fate and Speciation in the Environment ; grant no. 646002 ). SL, IH, SP and ZK received additional financial support from Fundação para a Ciência e a Tecnologia/Ministério da Ciência, Tecnologia e Ensino Superior ( FCT/MCTES ), through national funds, to CESAM ( UIDP/50017/2020 + UIDB/50017/2020 ). Also, this work was supported by FCT through a PhD grant to Sara Peixoto ( SFRH/BD/117738/2016 ). Publisher Copyright: © 2020 Elsevier Inc.

PY - 2020/12/15

Y1 - 2020/12/15

N2 - Soils might be a final sink for Ag2S nanoparticles (NPs). Still, there are limited data on their effects on soil bacterial communities (SBC). To bridge this gap, we investigated the effects of Ag2S NPs (10 mg kg−1 soil) on the structure and function of SBC in a terrestrial indoor mesocosm, using a multi-species design. During 28 days of exposure, the SBC function-related parameters were analysed in terms of enzymatic activity, community level physiological profile, culture of functional bacterial groups [phosphorous-solubilizing bacteria (P-SB) and heterotrophic bacteria (HB)], and SBC structure was analysed by 16S rRNA gene-targeted denaturing gradient gel electrophoresis. The SBC exposed to Ag2S NPs showed a significative decrease of functional parameters, such as β-glucosidase activity and L-arginine consumption, and increase of the acid phosphatase activity. At the structural level, significantly lower richness and diversity were detected, but at later exposure times compared to the AgNO3 treatment, likely because of a low dissolution rate of Ag2S NPs. In fact, stronger effects were observed in soils spiked with AgNO3, in both functional and structural parameters. Changes in SBC structure seem to negatively correlate with parameters related to phosphorous (acid phosphatase activity) and carbon cycling (abundance of HB, P-SB, and β-glucosidase activity). Our results indicate a significant effect of Ag2S NPs on SBC, specifically on parameters related to carbon and phosphorous cycling, at doses as low as 10 mg kg-1 soil. These effects were only observed after 28 days, highlighting the importance of long-term exposure experiments for slowly dissolving NPs.

AB - Soils might be a final sink for Ag2S nanoparticles (NPs). Still, there are limited data on their effects on soil bacterial communities (SBC). To bridge this gap, we investigated the effects of Ag2S NPs (10 mg kg−1 soil) on the structure and function of SBC in a terrestrial indoor mesocosm, using a multi-species design. During 28 days of exposure, the SBC function-related parameters were analysed in terms of enzymatic activity, community level physiological profile, culture of functional bacterial groups [phosphorous-solubilizing bacteria (P-SB) and heterotrophic bacteria (HB)], and SBC structure was analysed by 16S rRNA gene-targeted denaturing gradient gel electrophoresis. The SBC exposed to Ag2S NPs showed a significative decrease of functional parameters, such as β-glucosidase activity and L-arginine consumption, and increase of the acid phosphatase activity. At the structural level, significantly lower richness and diversity were detected, but at later exposure times compared to the AgNO3 treatment, likely because of a low dissolution rate of Ag2S NPs. In fact, stronger effects were observed in soils spiked with AgNO3, in both functional and structural parameters. Changes in SBC structure seem to negatively correlate with parameters related to phosphorous (acid phosphatase activity) and carbon cycling (abundance of HB, P-SB, and β-glucosidase activity). Our results indicate a significant effect of Ag2S NPs on SBC, specifically on parameters related to carbon and phosphorous cycling, at doses as low as 10 mg kg-1 soil. These effects were only observed after 28 days, highlighting the importance of long-term exposure experiments for slowly dissolving NPs.

KW - Community level physiological profile

KW - Denaturing gradient gel electrophoresis

KW - Indoor Mesocosm

KW - Silver sulfide nanoparticle

KW - Soil bacterial community

KW - Soil enzymatic activity

UR - http://www.scopus.com/inward/record.url?scp=85091768526&partnerID=8YFLogxK

U2 - 10.1016/j.ecoenv.2020.111405

DO - 10.1016/j.ecoenv.2020.111405

M3 - Article

C2 - 33010592

AN - SCOPUS:85091768526

VL - 206

JO - Ecotoxicology and Environmental Safety

JF - Ecotoxicology and Environmental Safety

SN - 0147-6513

M1 - 111405

ER -