TY - JOUR
T1 - Imaging workflow and calibration for CT-guided time-domain fluorescence tomography
AU - Tichauer, KM
AU - Holt, RW
AU - El-Ghussein, F
AU - Zhu, Qun
AU - Dehghani, Hamid
AU - Leblond, F
AU - Pogue, BW
PY - 2011/1/1
Y1 - 2011/1/1
N2 - In this study, several key optimization steps are outlined for a non-contact, time-correlated single photon counting small animal optical tomography system, using simultaneous collection of both fluorescence and transmittance data. The system is presented for time-domain image reconstruction in vivo, illustrating the sensitivity from single photon counting and the calibration steps needed to accurately process the data. In particular, laser time- and amplitude-referencing, detector and filter calibrations, and collection of a suitable instrument response function are all presented in the context of time-domain fluorescence tomography and a fully automated workflow is described. Preliminary phantom time-domain reconstructed images demonstrate the fidelity of the workflow for fluorescence tomography based on signal from multiple time gates.
AB - In this study, several key optimization steps are outlined for a non-contact, time-correlated single photon counting small animal optical tomography system, using simultaneous collection of both fluorescence and transmittance data. The system is presented for time-domain image reconstruction in vivo, illustrating the sensitivity from single photon counting and the calibration steps needed to accurately process the data. In particular, laser time- and amplitude-referencing, detector and filter calibrations, and collection of a suitable instrument response function are all presented in the context of time-domain fluorescence tomography and a fully automated workflow is described. Preliminary phantom time-domain reconstructed images demonstrate the fidelity of the workflow for fluorescence tomography based on signal from multiple time gates.
U2 - 10.1364/BOE.2.003021
DO - 10.1364/BOE.2.003021
M3 - Article
C2 - 22076264
SN - 2156-7085
VL - 2
SP - 3021
EP - 3036
JO - Biomedical Optics Express
JF - Biomedical Optics Express
IS - 11
ER -