Imaging Viral Behavior in Mammalian Cells with Self-Assembled Capsid-Quantum-Dot Hybrid Particles

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Imaging Viral Behavior in Mammalian Cells with Self-Assembled Capsid-Quantum-Dot Hybrid Particles. / Li, F; Zhang, ZP; Peng, J; Cui, ZQ; Pang, DW; Li, K; Wei, HP; Zhou, YF; Wen, Jikai; Zhang, XE.

In: Small, Vol. 5, No. 6, 01.03.2009, p. 718-726.

Research output: Contribution to journalArticle

Harvard

Li, F, Zhang, ZP, Peng, J, Cui, ZQ, Pang, DW, Li, K, Wei, HP, Zhou, YF, Wen, J & Zhang, XE 2009, 'Imaging Viral Behavior in Mammalian Cells with Self-Assembled Capsid-Quantum-Dot Hybrid Particles', Small, vol. 5, no. 6, pp. 718-726. https://doi.org/10.1002/smll.200801303

APA

Li, F., Zhang, ZP., Peng, J., Cui, ZQ., Pang, DW., Li, K., Wei, HP., Zhou, YF., Wen, J., & Zhang, XE. (2009). Imaging Viral Behavior in Mammalian Cells with Self-Assembled Capsid-Quantum-Dot Hybrid Particles. Small, 5(6), 718-726. https://doi.org/10.1002/smll.200801303

Vancouver

Author

Li, F ; Zhang, ZP ; Peng, J ; Cui, ZQ ; Pang, DW ; Li, K ; Wei, HP ; Zhou, YF ; Wen, Jikai ; Zhang, XE. / Imaging Viral Behavior in Mammalian Cells with Self-Assembled Capsid-Quantum-Dot Hybrid Particles. In: Small. 2009 ; Vol. 5, No. 6. pp. 718-726.

Bibtex

@article{e097106710c44f59ba4536b9ef51c4a8,
title = "Imaging Viral Behavior in Mammalian Cells with Self-Assembled Capsid-Quantum-Dot Hybrid Particles",
abstract = "Unique spectral properties of quantum dots (QDs) enable ultrasensitive and long-term biolabeling. Aiming to trace the infection, movement, and localization of viruses in living cells, QD-containing virus-like particles (VLPs) of simian virus 40 (SV40), termed SVLP-QDs, are constructed by in vitro self-assembly of the major capsid protein of SV40. SVLP-QDs show homogeneity in size (approximate to 24 nm), similarity in spectral properties to unencapsidated QDs, and considerable stability. When incubated with living cells, SVLP-QDs are shown to enter the cells by caveolar endocytosis, travel along the microtubules, and accumulate in the endoplasmic reticulum. This process mimics the early infection steps of SV40. This is the first paradigm of imaging viral behaviors with encapsidated QDs in living cells. The method may provide a new alternative for various purposes, such as tracing viruses or viral components, targeted nanoparticle delivery, and probing of drug delivery.",
keywords = "self-assembly, quantum dots, virus tracing, simian virus 40, hybrid materials",
author = "F Li and ZP Zhang and J Peng and ZQ Cui and DW Pang and K Li and HP Wei and YF Zhou and Jikai Wen and XE Zhang",
year = "2009",
month = mar,
day = "1",
doi = "10.1002/smll.200801303",
language = "English",
volume = "5",
pages = "718--726",
journal = "Small",
issn = "1613-6810",
publisher = "Wiley-VCH Verlag",
number = "6",

}

RIS

TY - JOUR

T1 - Imaging Viral Behavior in Mammalian Cells with Self-Assembled Capsid-Quantum-Dot Hybrid Particles

AU - Li, F

AU - Zhang, ZP

AU - Peng, J

AU - Cui, ZQ

AU - Pang, DW

AU - Li, K

AU - Wei, HP

AU - Zhou, YF

AU - Wen, Jikai

AU - Zhang, XE

PY - 2009/3/1

Y1 - 2009/3/1

N2 - Unique spectral properties of quantum dots (QDs) enable ultrasensitive and long-term biolabeling. Aiming to trace the infection, movement, and localization of viruses in living cells, QD-containing virus-like particles (VLPs) of simian virus 40 (SV40), termed SVLP-QDs, are constructed by in vitro self-assembly of the major capsid protein of SV40. SVLP-QDs show homogeneity in size (approximate to 24 nm), similarity in spectral properties to unencapsidated QDs, and considerable stability. When incubated with living cells, SVLP-QDs are shown to enter the cells by caveolar endocytosis, travel along the microtubules, and accumulate in the endoplasmic reticulum. This process mimics the early infection steps of SV40. This is the first paradigm of imaging viral behaviors with encapsidated QDs in living cells. The method may provide a new alternative for various purposes, such as tracing viruses or viral components, targeted nanoparticle delivery, and probing of drug delivery.

AB - Unique spectral properties of quantum dots (QDs) enable ultrasensitive and long-term biolabeling. Aiming to trace the infection, movement, and localization of viruses in living cells, QD-containing virus-like particles (VLPs) of simian virus 40 (SV40), termed SVLP-QDs, are constructed by in vitro self-assembly of the major capsid protein of SV40. SVLP-QDs show homogeneity in size (approximate to 24 nm), similarity in spectral properties to unencapsidated QDs, and considerable stability. When incubated with living cells, SVLP-QDs are shown to enter the cells by caveolar endocytosis, travel along the microtubules, and accumulate in the endoplasmic reticulum. This process mimics the early infection steps of SV40. This is the first paradigm of imaging viral behaviors with encapsidated QDs in living cells. The method may provide a new alternative for various purposes, such as tracing viruses or viral components, targeted nanoparticle delivery, and probing of drug delivery.

KW - self-assembly

KW - quantum dots

KW - virus tracing

KW - simian virus 40

KW - hybrid materials

U2 - 10.1002/smll.200801303

DO - 10.1002/smll.200801303

M3 - Article

C2 - 19242943

VL - 5

SP - 718

EP - 726

JO - Small

JF - Small

SN - 1613-6810

IS - 6

ER -