Identification of the pollen self-incompatibility determinant in Papaver rhoeas.

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Identification of the pollen self-incompatibility determinant in Papaver rhoeas. / Wheeler, MJ; de Graaf, BH; Hadjiosif, N; Perry, Ruth; Poulter, Natalie; Osman, Kim; Vatovec, S; Harper, A; Franklin, Frederick; Franklin Tong, Vernonica.

In: Nature, Vol. 459, No. 7249, 18.06.2009, p. 992-5.

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Wheeler, MJ ; de Graaf, BH ; Hadjiosif, N ; Perry, Ruth ; Poulter, Natalie ; Osman, Kim ; Vatovec, S ; Harper, A ; Franklin, Frederick ; Franklin Tong, Vernonica. / Identification of the pollen self-incompatibility determinant in Papaver rhoeas. In: Nature. 2009 ; Vol. 459, No. 7249. pp. 992-5.

Bibtex

@article{d955ff28ad194be78334cc4eaf319779,
title = "Identification of the pollen self-incompatibility determinant in Papaver rhoeas.",
abstract = "Higher plants produce seed through pollination, using specific interactions between pollen and pistil. Self-incompatibility is an important mechanism used in many species to prevent inbreeding; it is controlled by a multi-allelic S locus. 'Self' (incompatible) pollen is discriminated from 'non-self' (compatible) pollen by interaction of pollen and pistil S locus components, and is subsequently inhibited. In Papaver rhoeas, the pistil S locus product is a small protein that interacts with incompatible pollen, triggering a Ca(2+)-dependent signalling network, resulting in pollen inhibition and programmed cell death. Here we have cloned three alleles of a highly polymorphic pollen-expressed gene, PrpS (Papaver rhoeas pollen S), from Papaver and provide evidence that this encodes the pollen S locus determinant. PrpS is a single-copy gene linked to the pistil S gene (currently called S, but referred to hereafter as PrsS for Papaver rhoeas stigma S determinant). Sequence analysis indicates that PrsS and PrpS are equally ancient and probably co-evolved. PrpS encodes a novel approximately 20-kDa protein. Consistent with predictions that it is a transmembrane protein, PrpS is associated with the plasma membrane. We show that a predicted extracellular loop segment of PrpS interacts with PrsS and, using PrpS antisense oligonucleotides, we demonstrate that PrpS is involved in S-specific inhibition of incompatible pollen. Identification of PrpS represents a major advance in our understanding of the Papaver self-incompatibility system. As a novel cell-cell recognition determinant it contributes to the available information concerning the origins and evolution of cell-cell recognition systems involved in discrimination between self and non-self, which also include histocompatibility systems in primitive chordates and vertebrates.",
author = "MJ Wheeler and {de Graaf}, BH and N Hadjiosif and Ruth Perry and Natalie Poulter and Kim Osman and S Vatovec and A Harper and Frederick Franklin and {Franklin Tong}, Vernonica",
year = "2009",
month = jun,
day = "18",
doi = "10.1038/nature08027",
language = "English",
volume = "459",
pages = "992--5",
journal = "Nature",
issn = "0028-0836",
publisher = "Nature Publishing Group",
number = "7249",

}

RIS

TY - JOUR

T1 - Identification of the pollen self-incompatibility determinant in Papaver rhoeas.

AU - Wheeler, MJ

AU - de Graaf, BH

AU - Hadjiosif, N

AU - Perry, Ruth

AU - Poulter, Natalie

AU - Osman, Kim

AU - Vatovec, S

AU - Harper, A

AU - Franklin, Frederick

AU - Franklin Tong, Vernonica

PY - 2009/6/18

Y1 - 2009/6/18

N2 - Higher plants produce seed through pollination, using specific interactions between pollen and pistil. Self-incompatibility is an important mechanism used in many species to prevent inbreeding; it is controlled by a multi-allelic S locus. 'Self' (incompatible) pollen is discriminated from 'non-self' (compatible) pollen by interaction of pollen and pistil S locus components, and is subsequently inhibited. In Papaver rhoeas, the pistil S locus product is a small protein that interacts with incompatible pollen, triggering a Ca(2+)-dependent signalling network, resulting in pollen inhibition and programmed cell death. Here we have cloned three alleles of a highly polymorphic pollen-expressed gene, PrpS (Papaver rhoeas pollen S), from Papaver and provide evidence that this encodes the pollen S locus determinant. PrpS is a single-copy gene linked to the pistil S gene (currently called S, but referred to hereafter as PrsS for Papaver rhoeas stigma S determinant). Sequence analysis indicates that PrsS and PrpS are equally ancient and probably co-evolved. PrpS encodes a novel approximately 20-kDa protein. Consistent with predictions that it is a transmembrane protein, PrpS is associated with the plasma membrane. We show that a predicted extracellular loop segment of PrpS interacts with PrsS and, using PrpS antisense oligonucleotides, we demonstrate that PrpS is involved in S-specific inhibition of incompatible pollen. Identification of PrpS represents a major advance in our understanding of the Papaver self-incompatibility system. As a novel cell-cell recognition determinant it contributes to the available information concerning the origins and evolution of cell-cell recognition systems involved in discrimination between self and non-self, which also include histocompatibility systems in primitive chordates and vertebrates.

AB - Higher plants produce seed through pollination, using specific interactions between pollen and pistil. Self-incompatibility is an important mechanism used in many species to prevent inbreeding; it is controlled by a multi-allelic S locus. 'Self' (incompatible) pollen is discriminated from 'non-self' (compatible) pollen by interaction of pollen and pistil S locus components, and is subsequently inhibited. In Papaver rhoeas, the pistil S locus product is a small protein that interacts with incompatible pollen, triggering a Ca(2+)-dependent signalling network, resulting in pollen inhibition and programmed cell death. Here we have cloned three alleles of a highly polymorphic pollen-expressed gene, PrpS (Papaver rhoeas pollen S), from Papaver and provide evidence that this encodes the pollen S locus determinant. PrpS is a single-copy gene linked to the pistil S gene (currently called S, but referred to hereafter as PrsS for Papaver rhoeas stigma S determinant). Sequence analysis indicates that PrsS and PrpS are equally ancient and probably co-evolved. PrpS encodes a novel approximately 20-kDa protein. Consistent with predictions that it is a transmembrane protein, PrpS is associated with the plasma membrane. We show that a predicted extracellular loop segment of PrpS interacts with PrsS and, using PrpS antisense oligonucleotides, we demonstrate that PrpS is involved in S-specific inhibition of incompatible pollen. Identification of PrpS represents a major advance in our understanding of the Papaver self-incompatibility system. As a novel cell-cell recognition determinant it contributes to the available information concerning the origins and evolution of cell-cell recognition systems involved in discrimination between self and non-self, which also include histocompatibility systems in primitive chordates and vertebrates.

U2 - 10.1038/nature08027

DO - 10.1038/nature08027

M3 - Article

C2 - 19483678

VL - 459

SP - 992

EP - 995

JO - Nature

JF - Nature

SN - 0028-0836

IS - 7249

ER -