Identification of RNA-protein interactions through in-vitro RNA pull-down assays
Research output: Chapter in Book/Report/Conference proceeding › Chapter (peer-reviewed) › peer-review
Colleges, School and Institutes
Recent advances in next generation sequencing have revealed that majority of the human genome is transcribed into long and short RNA (ncRNA) transcripts. Many ncRNAs function by interacting with proteins and forming regulatory complexes. RNA-protein interactions are vital in controlling core cellular processes like transcription and translation. Therefore identifying proteins that interact with ncRNAs is central to deciphering ncRNA functions. Here we describe a RNA-protein pull-down assay, which enable the identification of proteins that interact with a RNA under study. As an example we describe pull-down of proteins interacting with ncRNA XIST, which assists in the recruitment of the polycomb repressive complex-2 (PRC2) and drives X-chromosomal inactivation.
|Title of host publication||Identification of RNA-protein interactions through in-vitro RNA pull-down assays|
|Editors||Chiara Lanzuolo, Beatrice Bodega|
|Publication status||Accepted/In press - 8 Sep 2016|
|Name||Methods in molecular biology|