Identification of RNA-protein interactions through in-vitro RNA pull-down assays

Research output: Chapter in Book/Report/Conference proceedingChapter (peer-reviewed)peer-review

Authors

Colleges, School and Institutes

Abstract

Recent advances in next generation sequencing have revealed that majority of the human genome is transcribed into long and short RNA (ncRNA) transcripts. Many ncRNAs function by interacting with proteins and forming regulatory complexes. RNA-protein interactions are vital in controlling core cellular processes like transcription and translation. Therefore identifying proteins that interact with ncRNAs is central to deciphering ncRNA functions. Here we describe a RNA-protein pull-down assay, which enable the identification of proteins that interact with a RNA under study. As an example we describe pull-down of proteins interacting with ncRNA XIST, which assists in the recruitment of the polycomb repressive complex-2 (PRC2) and drives X-chromosomal inactivation.

Details

Original languageEnglish
Title of host publicationIdentification of RNA-protein interactions through in-vitro RNA pull-down assays
EditorsChiara Lanzuolo, Beatrice Bodega
Publication statusAccepted/In press - 8 Sep 2016

Publication series

NameMethods in molecular biology
PublisherHumana Press
Volume 1480
ISSN (Print)1064-3745