Identification of Epstein-Barr Virus replication proteins in Burkitt's lymphoma cells

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Identification of Epstein-Barr Virus replication proteins in Burkitt's lymphoma cells. / Traylen, Chris; Ramasubramanyan, Sharada; Zuo, Jianmin; Rowe, Martin; Almohammad, Rajaei; Heesom, Kate; Sweet, Steve M M; Matthews, David A; Sinclair, Alison J.

In: Pathogens, Vol. 4, No. 4, 25.10.2015, p. 739-751.

Research output: Contribution to journalArticlepeer-review

Harvard

Traylen, C, Ramasubramanyan, S, Zuo, J, Rowe, M, Almohammad, R, Heesom, K, Sweet, SMM, Matthews, DA & Sinclair, AJ 2015, 'Identification of Epstein-Barr Virus replication proteins in Burkitt's lymphoma cells', Pathogens, vol. 4, no. 4, pp. 739-751. https://doi.org/10.3390/pathogens4040739

APA

Traylen, C., Ramasubramanyan, S., Zuo, J., Rowe, M., Almohammad, R., Heesom, K., Sweet, S. M. M., Matthews, D. A., & Sinclair, A. J. (2015). Identification of Epstein-Barr Virus replication proteins in Burkitt's lymphoma cells. Pathogens, 4(4), 739-751. https://doi.org/10.3390/pathogens4040739

Vancouver

Author

Traylen, Chris ; Ramasubramanyan, Sharada ; Zuo, Jianmin ; Rowe, Martin ; Almohammad, Rajaei ; Heesom, Kate ; Sweet, Steve M M ; Matthews, David A ; Sinclair, Alison J. / Identification of Epstein-Barr Virus replication proteins in Burkitt's lymphoma cells. In: Pathogens. 2015 ; Vol. 4, No. 4. pp. 739-751.

Bibtex

@article{472b430e896d406aab7beaa714c095a1,
title = "Identification of Epstein-Barr Virus replication proteins in Burkitt's lymphoma cells",
abstract = "The working model to describe the mechanisms used to replicate the cancer-associated virus Epstein-Barr virus (EBV) is partly derived from comparisons with other members of the Herpes virus family. Many genes within the EBV genome are homologous across the herpes virus family. Published transcriptome data for the EBV genome during its lytic replication cycle show extensive transcription, but the identification of the proteins is limited. We have taken a global proteomics approach to identify viral proteins that are expressed during the EBV lytic replication cycle. We combined an enrichment method to isolate cells undergoing EBV lytic replication with SILAC-labeling coupled to mass-spectrometry and identified viral and host proteins expressed during the OPEN ACCESS Pathogens 2015, 4 740 EBV lytic replication cycle. Amongst the most frequently identified viral proteins are two components of the DNA replication machinery, the single strand DNA binding protein BALF2, DNA polymerase accessory protein BMRF1 and both subunits of the viral ribonucleoside-diphosphate reductase enzyme (BORF2 and BaRF1). An additional 42 EBV lytic cycle proteins were also detected. This provides proteomic identification for many EBV lytic replication cycle proteins and also identifies post-translational modifications.",
keywords = "virus, Epstein-Barr, herpes, proteome, replication, cancer",
author = "Chris Traylen and Sharada Ramasubramanyan and Jianmin Zuo and Martin Rowe and Rajaei Almohammad and Kate Heesom and Sweet, {Steve M M} and Matthews, {David A} and Sinclair, {Alison J}",
year = "2015",
month = oct,
day = "25",
doi = "10.3390/pathogens4040739",
language = "English",
volume = "4",
pages = "739--751",
journal = "Pathogens",
issn = "2076-0817",
publisher = "MDPI",
number = "4",

}

RIS

TY - JOUR

T1 - Identification of Epstein-Barr Virus replication proteins in Burkitt's lymphoma cells

AU - Traylen, Chris

AU - Ramasubramanyan, Sharada

AU - Zuo, Jianmin

AU - Rowe, Martin

AU - Almohammad, Rajaei

AU - Heesom, Kate

AU - Sweet, Steve M M

AU - Matthews, David A

AU - Sinclair, Alison J

PY - 2015/10/25

Y1 - 2015/10/25

N2 - The working model to describe the mechanisms used to replicate the cancer-associated virus Epstein-Barr virus (EBV) is partly derived from comparisons with other members of the Herpes virus family. Many genes within the EBV genome are homologous across the herpes virus family. Published transcriptome data for the EBV genome during its lytic replication cycle show extensive transcription, but the identification of the proteins is limited. We have taken a global proteomics approach to identify viral proteins that are expressed during the EBV lytic replication cycle. We combined an enrichment method to isolate cells undergoing EBV lytic replication with SILAC-labeling coupled to mass-spectrometry and identified viral and host proteins expressed during the OPEN ACCESS Pathogens 2015, 4 740 EBV lytic replication cycle. Amongst the most frequently identified viral proteins are two components of the DNA replication machinery, the single strand DNA binding protein BALF2, DNA polymerase accessory protein BMRF1 and both subunits of the viral ribonucleoside-diphosphate reductase enzyme (BORF2 and BaRF1). An additional 42 EBV lytic cycle proteins were also detected. This provides proteomic identification for many EBV lytic replication cycle proteins and also identifies post-translational modifications.

AB - The working model to describe the mechanisms used to replicate the cancer-associated virus Epstein-Barr virus (EBV) is partly derived from comparisons with other members of the Herpes virus family. Many genes within the EBV genome are homologous across the herpes virus family. Published transcriptome data for the EBV genome during its lytic replication cycle show extensive transcription, but the identification of the proteins is limited. We have taken a global proteomics approach to identify viral proteins that are expressed during the EBV lytic replication cycle. We combined an enrichment method to isolate cells undergoing EBV lytic replication with SILAC-labeling coupled to mass-spectrometry and identified viral and host proteins expressed during the OPEN ACCESS Pathogens 2015, 4 740 EBV lytic replication cycle. Amongst the most frequently identified viral proteins are two components of the DNA replication machinery, the single strand DNA binding protein BALF2, DNA polymerase accessory protein BMRF1 and both subunits of the viral ribonucleoside-diphosphate reductase enzyme (BORF2 and BaRF1). An additional 42 EBV lytic cycle proteins were also detected. This provides proteomic identification for many EBV lytic replication cycle proteins and also identifies post-translational modifications.

KW - virus

KW - Epstein-Barr

KW - herpes

KW - proteome

KW - replication

KW - cancer

U2 - 10.3390/pathogens4040739

DO - 10.3390/pathogens4040739

M3 - Article

C2 - 26529022

VL - 4

SP - 739

EP - 751

JO - Pathogens

JF - Pathogens

SN - 2076-0817

IS - 4

ER -