Identification of a novel, actin-rich structure, the actin nodule, in the early stages of platelet spreading.

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Identification of a novel, actin-rich structure, the actin nodule, in the early stages of platelet spreading. / Calaminus, Simon; Thomas, Steven; McCarty, OJ; Machesky, LM; Watson, Steve.

In: Journal of Thrombosis and Haemostasis, Vol. 6, No. 11, 01.11.2008, p. 1944-52.

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@article{9201b19c7d904d8d8d1687863576ef60,
title = "Identification of a novel, actin-rich structure, the actin nodule, in the early stages of platelet spreading.",
abstract = "BACKGROUND: During platelet spreading, the actin cytoskeleton undergoes marked changes, forming filopodia, lamellipodia and stress fibres. In the present study, we report the identification of a novel actin-rich structure, termed an actin nodule, which appears prior to lamellipodia and stress fibre formation. METHODS: Platelet spreading was monitored using human platelets and mouse GFP-actin platelets using real-time and end-point DIC, and fluorescent and electron microscopy (EM). RESULTS: We identified a small, novel actin structure, the actin nodule, in the early stages of adhesion and spreading, which we hypothesize to be a precursor of lamellipodia and stress fibres. Nodule formation shows an inverse correlation to Rho kinase and myosin-II activity, is independent of PI3-kinase, but dependent on Src kinase activity. Actin nodules contain multiple proteins, including Arp2/3, Fyn, Rac, and beta1- and beta3- integrins, but not Src. EM analysis revealed that actin filaments extend in all directions from the nodules. Actin nodules are present on multiple matrices, including fibrinogen, laminin and VWF + botrocetin. CONCLUSION: This work identifies a novel platelet actin structure, which we propose is a precursor to both lamellipodia and stress fibres and acts to drive platelet spreading.",
keywords = "Src kinases, platelet spreading, Rho kinase, Arp2/3, actin cytoskeleton",
author = "Simon Calaminus and Steven Thomas and OJ McCarty and LM Machesky and Steve Watson",
year = "2008",
month = nov,
day = "1",
doi = "10.1111/j.1538-7836.2008.03141.x",
language = "English",
volume = "6",
pages = "1944--52",
journal = "Journal of Thrombosis and Haemostasis",
issn = "1538-7933",
publisher = "Wiley",
number = "11",

}

RIS

TY - JOUR

T1 - Identification of a novel, actin-rich structure, the actin nodule, in the early stages of platelet spreading.

AU - Calaminus, Simon

AU - Thomas, Steven

AU - McCarty, OJ

AU - Machesky, LM

AU - Watson, Steve

PY - 2008/11/1

Y1 - 2008/11/1

N2 - BACKGROUND: During platelet spreading, the actin cytoskeleton undergoes marked changes, forming filopodia, lamellipodia and stress fibres. In the present study, we report the identification of a novel actin-rich structure, termed an actin nodule, which appears prior to lamellipodia and stress fibre formation. METHODS: Platelet spreading was monitored using human platelets and mouse GFP-actin platelets using real-time and end-point DIC, and fluorescent and electron microscopy (EM). RESULTS: We identified a small, novel actin structure, the actin nodule, in the early stages of adhesion and spreading, which we hypothesize to be a precursor of lamellipodia and stress fibres. Nodule formation shows an inverse correlation to Rho kinase and myosin-II activity, is independent of PI3-kinase, but dependent on Src kinase activity. Actin nodules contain multiple proteins, including Arp2/3, Fyn, Rac, and beta1- and beta3- integrins, but not Src. EM analysis revealed that actin filaments extend in all directions from the nodules. Actin nodules are present on multiple matrices, including fibrinogen, laminin and VWF + botrocetin. CONCLUSION: This work identifies a novel platelet actin structure, which we propose is a precursor to both lamellipodia and stress fibres and acts to drive platelet spreading.

AB - BACKGROUND: During platelet spreading, the actin cytoskeleton undergoes marked changes, forming filopodia, lamellipodia and stress fibres. In the present study, we report the identification of a novel actin-rich structure, termed an actin nodule, which appears prior to lamellipodia and stress fibre formation. METHODS: Platelet spreading was monitored using human platelets and mouse GFP-actin platelets using real-time and end-point DIC, and fluorescent and electron microscopy (EM). RESULTS: We identified a small, novel actin structure, the actin nodule, in the early stages of adhesion and spreading, which we hypothesize to be a precursor of lamellipodia and stress fibres. Nodule formation shows an inverse correlation to Rho kinase and myosin-II activity, is independent of PI3-kinase, but dependent on Src kinase activity. Actin nodules contain multiple proteins, including Arp2/3, Fyn, Rac, and beta1- and beta3- integrins, but not Src. EM analysis revealed that actin filaments extend in all directions from the nodules. Actin nodules are present on multiple matrices, including fibrinogen, laminin and VWF + botrocetin. CONCLUSION: This work identifies a novel platelet actin structure, which we propose is a precursor to both lamellipodia and stress fibres and acts to drive platelet spreading.

KW - Src kinases

KW - platelet spreading

KW - Rho kinase

KW - Arp2/3

KW - actin cytoskeleton

U2 - 10.1111/j.1538-7836.2008.03141.x

DO - 10.1111/j.1538-7836.2008.03141.x

M3 - Article

C2 - 18761725

VL - 6

SP - 1944

EP - 1952

JO - Journal of Thrombosis and Haemostasis

JF - Journal of Thrombosis and Haemostasis

SN - 1538-7933

IS - 11

ER -