Identification of a new subset of lymph node stromal cells involved in regulating plasma cell homeostasis

Research output: Contribution to journalArticle

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Identification of a new subset of lymph node stromal cells involved in regulating plasma cell homeostasis. / Huang, Hsin-Ying; Rivas-Caicedo, Ana; Renevey, François; Cannelle, Hélène; Peranzoni, Elisa; Scarpellino, Leonardo; Hardie, Debbie; Pommier, Arnaud; Schaeuble, Karin; Favre, Stéphanie; Vogt, Tobias K.; Arenzana-Seisdedos, Fernando; Schneider, Pascal; Buckley, Christopher; Donnadieu, Emmanuel; Luther, Sanjiv A.

In: Proceedings of the National Academy of Sciences of the United States of America, 02.07.2018.

Research output: Contribution to journalArticle

Harvard

Huang, H-Y, Rivas-Caicedo, A, Renevey, F, Cannelle, H, Peranzoni, E, Scarpellino, L, Hardie, D, Pommier, A, Schaeuble, K, Favre, S, Vogt, TK, Arenzana-Seisdedos, F, Schneider, P, Buckley, C, Donnadieu, E & Luther, SA 2018, 'Identification of a new subset of lymph node stromal cells involved in regulating plasma cell homeostasis', Proceedings of the National Academy of Sciences of the United States of America. https://doi.org/10.1073/pnas.1712628115

APA

Huang, H-Y., Rivas-Caicedo, A., Renevey, F., Cannelle, H., Peranzoni, E., Scarpellino, L., Hardie, D., Pommier, A., Schaeuble, K., Favre, S., Vogt, T. K., Arenzana-Seisdedos, F., Schneider, P., Buckley, C., Donnadieu, E., & Luther, S. A. (2018). Identification of a new subset of lymph node stromal cells involved in regulating plasma cell homeostasis. Proceedings of the National Academy of Sciences of the United States of America. https://doi.org/10.1073/pnas.1712628115

Vancouver

Author

Huang, Hsin-Ying ; Rivas-Caicedo, Ana ; Renevey, François ; Cannelle, Hélène ; Peranzoni, Elisa ; Scarpellino, Leonardo ; Hardie, Debbie ; Pommier, Arnaud ; Schaeuble, Karin ; Favre, Stéphanie ; Vogt, Tobias K. ; Arenzana-Seisdedos, Fernando ; Schneider, Pascal ; Buckley, Christopher ; Donnadieu, Emmanuel ; Luther, Sanjiv A. / Identification of a new subset of lymph node stromal cells involved in regulating plasma cell homeostasis. In: Proceedings of the National Academy of Sciences of the United States of America. 2018.

Bibtex

@article{c0e536953cbe49d6b7878a601283d73c,
title = "Identification of a new subset of lymph node stromal cells involved in regulating plasma cell homeostasis",
abstract = "Antibody-secreting plasma cells (PC) arise rapidly during adaptive immunity to control infections. The early PC are retained within the reactive lymphoid organ where their localization and homeostasis relies on extrinsic factors, presumably produced by local niche cells. While myeloid cells have been proposed to form those niches, the contribution by co-localizing stromal cells has remained unclear. Here, we characterized a subset of fibroblastic reticular cells (FRC) that forms a dense meshwork throughout medullary cords of lymph nodes (LN) where PC reside. This medullary FRC type is shown to be anatomically, phenotypically and functionally distinct from T zone FRC, both in mice and humans. By using static and dynamic imaging approaches, we provide evidence that medullary FRC are the main cell type in contact with PC guiding them in their migration. Medullary FRC also represent a major local source of the PC survival factors IL-6, BAFF and CXCL12, besides producing also APRIL. In vitro, medullary FRC alone or in combination with macrophages promote PC survival while other LN cell types do not have this property. Thus, we propose that this new FRC subset, together with medullary macrophages, forms PC survival niches within the LN medulla, and thereby helps promoting the rapid development of humoral immunity which is critical in limiting early pathogen spread.",
keywords = "FRC subsets , lymph node medulla, extrafollicular B cell differentiation , plasma cell survival, humoral response",
author = "Hsin-Ying Huang and Ana Rivas-Caicedo and Fran{\c c}ois Renevey and H{\'e}l{\`e}ne Cannelle and Elisa Peranzoni and Leonardo Scarpellino and Debbie Hardie and Arnaud Pommier and Karin Schaeuble and St{\'e}phanie Favre and Vogt, {Tobias K.} and Fernando Arenzana-Seisdedos and Pascal Schneider and Christopher Buckley and Emmanuel Donnadieu and Luther, {Sanjiv A.}",
year = "2018",
month = jul
day = "2",
doi = "10.1073/pnas.1712628115",
language = "English",
journal = "National Academy of Sciences. Proceedings",
issn = "1091-6490",
publisher = "National Academy of Sciences",

}

RIS

TY - JOUR

T1 - Identification of a new subset of lymph node stromal cells involved in regulating plasma cell homeostasis

AU - Huang, Hsin-Ying

AU - Rivas-Caicedo, Ana

AU - Renevey, François

AU - Cannelle, Hélène

AU - Peranzoni, Elisa

AU - Scarpellino, Leonardo

AU - Hardie, Debbie

AU - Pommier, Arnaud

AU - Schaeuble, Karin

AU - Favre, Stéphanie

AU - Vogt, Tobias K.

AU - Arenzana-Seisdedos, Fernando

AU - Schneider, Pascal

AU - Buckley, Christopher

AU - Donnadieu, Emmanuel

AU - Luther, Sanjiv A.

PY - 2018/7/2

Y1 - 2018/7/2

N2 - Antibody-secreting plasma cells (PC) arise rapidly during adaptive immunity to control infections. The early PC are retained within the reactive lymphoid organ where their localization and homeostasis relies on extrinsic factors, presumably produced by local niche cells. While myeloid cells have been proposed to form those niches, the contribution by co-localizing stromal cells has remained unclear. Here, we characterized a subset of fibroblastic reticular cells (FRC) that forms a dense meshwork throughout medullary cords of lymph nodes (LN) where PC reside. This medullary FRC type is shown to be anatomically, phenotypically and functionally distinct from T zone FRC, both in mice and humans. By using static and dynamic imaging approaches, we provide evidence that medullary FRC are the main cell type in contact with PC guiding them in their migration. Medullary FRC also represent a major local source of the PC survival factors IL-6, BAFF and CXCL12, besides producing also APRIL. In vitro, medullary FRC alone or in combination with macrophages promote PC survival while other LN cell types do not have this property. Thus, we propose that this new FRC subset, together with medullary macrophages, forms PC survival niches within the LN medulla, and thereby helps promoting the rapid development of humoral immunity which is critical in limiting early pathogen spread.

AB - Antibody-secreting plasma cells (PC) arise rapidly during adaptive immunity to control infections. The early PC are retained within the reactive lymphoid organ where their localization and homeostasis relies on extrinsic factors, presumably produced by local niche cells. While myeloid cells have been proposed to form those niches, the contribution by co-localizing stromal cells has remained unclear. Here, we characterized a subset of fibroblastic reticular cells (FRC) that forms a dense meshwork throughout medullary cords of lymph nodes (LN) where PC reside. This medullary FRC type is shown to be anatomically, phenotypically and functionally distinct from T zone FRC, both in mice and humans. By using static and dynamic imaging approaches, we provide evidence that medullary FRC are the main cell type in contact with PC guiding them in their migration. Medullary FRC also represent a major local source of the PC survival factors IL-6, BAFF and CXCL12, besides producing also APRIL. In vitro, medullary FRC alone or in combination with macrophages promote PC survival while other LN cell types do not have this property. Thus, we propose that this new FRC subset, together with medullary macrophages, forms PC survival niches within the LN medulla, and thereby helps promoting the rapid development of humoral immunity which is critical in limiting early pathogen spread.

KW - FRC subsets

KW - lymph node medulla

KW - extrafollicular B cell differentiation

KW - plasma cell survival

KW - humoral response

U2 - 10.1073/pnas.1712628115

DO - 10.1073/pnas.1712628115

M3 - Article

JO - National Academy of Sciences. Proceedings

JF - National Academy of Sciences. Proceedings

SN - 1091-6490

ER -