Identification of a DNA nonhomologous end-joining complex in bacteria

Research output: Contribution to journalArticle

Standard

Identification of a DNA nonhomologous end-joining complex in bacteria. / Weller, GR; Kysela, Boris; Roy, R; Tonkin, LM; Scanlan, E; Della, M; Devine, SK; Day, JP; Wilkinson, A; di Fagagna, F; Devine, KM; Bowater, RP; Jeggo, AP; Jackson, SP; Doherty, AJ.

In: Science, Vol. 297, No. 5587, 06.09.2002, p. 1686-9.

Research output: Contribution to journalArticle

Harvard

Weller, GR, Kysela, B, Roy, R, Tonkin, LM, Scanlan, E, Della, M, Devine, SK, Day, JP, Wilkinson, A, di Fagagna, F, Devine, KM, Bowater, RP, Jeggo, AP, Jackson, SP & Doherty, AJ 2002, 'Identification of a DNA nonhomologous end-joining complex in bacteria', Science, vol. 297, no. 5587, pp. 1686-9. https://doi.org/10.1126/science.1074584

APA

Weller, GR., Kysela, B., Roy, R., Tonkin, LM., Scanlan, E., Della, M., Devine, SK., Day, JP., Wilkinson, A., di Fagagna, F., Devine, KM., Bowater, RP., Jeggo, AP., Jackson, SP., & Doherty, AJ. (2002). Identification of a DNA nonhomologous end-joining complex in bacteria. Science, 297(5587), 1686-9. https://doi.org/10.1126/science.1074584

Vancouver

Weller GR, Kysela B, Roy R, Tonkin LM, Scanlan E, Della M et al. Identification of a DNA nonhomologous end-joining complex in bacteria. Science. 2002 Sep 6;297(5587):1686-9. https://doi.org/10.1126/science.1074584

Author

Weller, GR ; Kysela, Boris ; Roy, R ; Tonkin, LM ; Scanlan, E ; Della, M ; Devine, SK ; Day, JP ; Wilkinson, A ; di Fagagna, F ; Devine, KM ; Bowater, RP ; Jeggo, AP ; Jackson, SP ; Doherty, AJ. / Identification of a DNA nonhomologous end-joining complex in bacteria. In: Science. 2002 ; Vol. 297, No. 5587. pp. 1686-9.

Bibtex

@article{c906e11aa42f456a9c978164905b2281,
title = "Identification of a DNA nonhomologous end-joining complex in bacteria",
abstract = "In eukaryotic cells, double-strand breaks (DSBs) in DNA are generally repaired by the pathway of homologous recombination or by DNA nonhomologous end joining (NHEJ). Both pathways have been highly conserved throughout eukaryotic evolution, but no equivalent NHEJ system has been identified in prokaryotes. The NHEJ pathway requires a DNA end-binding component called Ku. We have identified bacterial Ku homologs and show that these proteins retain the biochemical characteristics of the eukaryotic Ku heterodimer. Furthermore, we show that bacterial Ku specifically recruits DNA ligase to DNA ends and stimulates DNA ligation. Loss of these proteins leads to hypersensitivity to ionizing radiation in Bacillus subtilis. These data provide evidence that many bacteria possess a DNA DSB repair apparatus that shares many features with the NHEJ system of eukarya and suggest that this DNA repair pathway arose before the prokaryotic and eukaryotic lineages diverged.",
author = "GR Weller and Boris Kysela and R Roy and LM Tonkin and E Scanlan and M Della and SK Devine and JP Day and A Wilkinson and {di Fagagna}, F and KM Devine and RP Bowater and AP Jeggo and SP Jackson and AJ Doherty",
year = "2002",
month = sep,
day = "6",
doi = "10.1126/science.1074584",
language = "English",
volume = "297",
pages = "1686--9",
journal = "Science",
issn = "0036-8075",
publisher = "American Association for the Advancement of Science",
number = "5587",

}

RIS

TY - JOUR

T1 - Identification of a DNA nonhomologous end-joining complex in bacteria

AU - Weller, GR

AU - Kysela, Boris

AU - Roy, R

AU - Tonkin, LM

AU - Scanlan, E

AU - Della, M

AU - Devine, SK

AU - Day, JP

AU - Wilkinson, A

AU - di Fagagna, F

AU - Devine, KM

AU - Bowater, RP

AU - Jeggo, AP

AU - Jackson, SP

AU - Doherty, AJ

PY - 2002/9/6

Y1 - 2002/9/6

N2 - In eukaryotic cells, double-strand breaks (DSBs) in DNA are generally repaired by the pathway of homologous recombination or by DNA nonhomologous end joining (NHEJ). Both pathways have been highly conserved throughout eukaryotic evolution, but no equivalent NHEJ system has been identified in prokaryotes. The NHEJ pathway requires a DNA end-binding component called Ku. We have identified bacterial Ku homologs and show that these proteins retain the biochemical characteristics of the eukaryotic Ku heterodimer. Furthermore, we show that bacterial Ku specifically recruits DNA ligase to DNA ends and stimulates DNA ligation. Loss of these proteins leads to hypersensitivity to ionizing radiation in Bacillus subtilis. These data provide evidence that many bacteria possess a DNA DSB repair apparatus that shares many features with the NHEJ system of eukarya and suggest that this DNA repair pathway arose before the prokaryotic and eukaryotic lineages diverged.

AB - In eukaryotic cells, double-strand breaks (DSBs) in DNA are generally repaired by the pathway of homologous recombination or by DNA nonhomologous end joining (NHEJ). Both pathways have been highly conserved throughout eukaryotic evolution, but no equivalent NHEJ system has been identified in prokaryotes. The NHEJ pathway requires a DNA end-binding component called Ku. We have identified bacterial Ku homologs and show that these proteins retain the biochemical characteristics of the eukaryotic Ku heterodimer. Furthermore, we show that bacterial Ku specifically recruits DNA ligase to DNA ends and stimulates DNA ligation. Loss of these proteins leads to hypersensitivity to ionizing radiation in Bacillus subtilis. These data provide evidence that many bacteria possess a DNA DSB repair apparatus that shares many features with the NHEJ system of eukarya and suggest that this DNA repair pathway arose before the prokaryotic and eukaryotic lineages diverged.

UR - http://www.scopus.com/inward/record.url?scp=0037031655&partnerID=8YFLogxK

U2 - 10.1126/science.1074584

DO - 10.1126/science.1074584

M3 - Article

C2 - 12215643

VL - 297

SP - 1686

EP - 1689

JO - Science

JF - Science

SN - 0036-8075

IS - 5587

ER -