Identification of 5-HT3A and 5-HT3B receptor subunits in human hippocampus

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Identification of 5-HT3A and 5-HT3B receptor subunits in human hippocampus. / Brady, Catherine; Dover, Terri; Massoura, AN; Princivalle, Alessandra; Hope, Anthony; Barnes, Nicholas.

In: Neuropharmacology, Vol. 52, No. 5, 01.04.2007, p. 1284-1290.

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@article{9d4f19e18fc44dfa8122141a0357bcbd,
title = "Identification of 5-HT3A and 5-HT3B receptor subunits in human hippocampus",
abstract = "The pentameric 5-HT(3) receptor complex is a ligand-gated ion channel that mediates fast synaptic transmission in the brain. Expression of two subunits (5-HT(3A) and 5-HT(3B) subunits) gives rise to at least two receptor isoforms (homomeric 5-HT(3A) and heteromeric 5-HT(3A/3B) receptors), which differ in their biophysical characteristics, although expression of these proteins has not been investigated in human brain. The expression of h5-HT(3A) and 5-HT(3B) subunits in the human hippocampus was investigated using selective polyclonal antibodies (SDS-PAGE/Western blotting, immunohistochemistry), with expression of each subunit verified by PCR detection of subunit transcripts. 5-HT(3A) and 5-HT(3B) subunit immunoreactivity was identified within the human hippocampus. The cellular pattern of expression for each subunit was similar, with predominant immunoreactivity associated with pyramidal neurones in CA fields 2 and 3, and also the relatively large neurones within the hilus (CA4 field). Transcripts for each subunit were also identified in human hippocampal tissue. These findings indicate that human hippocampal neurones are capable of forming at least two, functionally different, isoforms of the 5-HT(3) receptor. Furthermore the expression pattern of 5-HT(3A) and 5-HT(3B) subunits in human hippocampus appears to differ with the rodent counterpart, which may underlie the differences in some of the behavioural effects of 5-HT(3) receptor antagonists between these species.",
keywords = "serotonin, 5-HT3 receptor, human hippocampus, 5-HT3B subunit, 5-HT3A subunit, immunoreactivity",
author = "Catherine Brady and Terri Dover and AN Massoura and Alessandra Princivalle and Anthony Hope and Nicholas Barnes",
year = "2007",
month = apr,
day = "1",
doi = "10.1016/j.neuropharm.2007.01.009",
language = "English",
volume = "52",
pages = "1284--1290",
journal = "Neuropharmacology",
issn = "0028-3908",
publisher = "Elsevier",
number = "5",

}

RIS

TY - JOUR

T1 - Identification of 5-HT3A and 5-HT3B receptor subunits in human hippocampus

AU - Brady, Catherine

AU - Dover, Terri

AU - Massoura, AN

AU - Princivalle, Alessandra

AU - Hope, Anthony

AU - Barnes, Nicholas

PY - 2007/4/1

Y1 - 2007/4/1

N2 - The pentameric 5-HT(3) receptor complex is a ligand-gated ion channel that mediates fast synaptic transmission in the brain. Expression of two subunits (5-HT(3A) and 5-HT(3B) subunits) gives rise to at least two receptor isoforms (homomeric 5-HT(3A) and heteromeric 5-HT(3A/3B) receptors), which differ in their biophysical characteristics, although expression of these proteins has not been investigated in human brain. The expression of h5-HT(3A) and 5-HT(3B) subunits in the human hippocampus was investigated using selective polyclonal antibodies (SDS-PAGE/Western blotting, immunohistochemistry), with expression of each subunit verified by PCR detection of subunit transcripts. 5-HT(3A) and 5-HT(3B) subunit immunoreactivity was identified within the human hippocampus. The cellular pattern of expression for each subunit was similar, with predominant immunoreactivity associated with pyramidal neurones in CA fields 2 and 3, and also the relatively large neurones within the hilus (CA4 field). Transcripts for each subunit were also identified in human hippocampal tissue. These findings indicate that human hippocampal neurones are capable of forming at least two, functionally different, isoforms of the 5-HT(3) receptor. Furthermore the expression pattern of 5-HT(3A) and 5-HT(3B) subunits in human hippocampus appears to differ with the rodent counterpart, which may underlie the differences in some of the behavioural effects of 5-HT(3) receptor antagonists between these species.

AB - The pentameric 5-HT(3) receptor complex is a ligand-gated ion channel that mediates fast synaptic transmission in the brain. Expression of two subunits (5-HT(3A) and 5-HT(3B) subunits) gives rise to at least two receptor isoforms (homomeric 5-HT(3A) and heteromeric 5-HT(3A/3B) receptors), which differ in their biophysical characteristics, although expression of these proteins has not been investigated in human brain. The expression of h5-HT(3A) and 5-HT(3B) subunits in the human hippocampus was investigated using selective polyclonal antibodies (SDS-PAGE/Western blotting, immunohistochemistry), with expression of each subunit verified by PCR detection of subunit transcripts. 5-HT(3A) and 5-HT(3B) subunit immunoreactivity was identified within the human hippocampus. The cellular pattern of expression for each subunit was similar, with predominant immunoreactivity associated with pyramidal neurones in CA fields 2 and 3, and also the relatively large neurones within the hilus (CA4 field). Transcripts for each subunit were also identified in human hippocampal tissue. These findings indicate that human hippocampal neurones are capable of forming at least two, functionally different, isoforms of the 5-HT(3) receptor. Furthermore the expression pattern of 5-HT(3A) and 5-HT(3B) subunits in human hippocampus appears to differ with the rodent counterpart, which may underlie the differences in some of the behavioural effects of 5-HT(3) receptor antagonists between these species.

KW - serotonin

KW - 5-HT3 receptor

KW - human hippocampus

KW - 5-HT3B subunit

KW - 5-HT3A subunit

KW - immunoreactivity

U2 - 10.1016/j.neuropharm.2007.01.009

DO - 10.1016/j.neuropharm.2007.01.009

M3 - Article

C2 - 17327132

VL - 52

SP - 1284

EP - 1290

JO - Neuropharmacology

JF - Neuropharmacology

SN - 0028-3908

IS - 5

ER -