Human insulin gene enhancer-binding proteins in pancreatic alpha and beta cell lines

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Human insulin gene enhancer-binding proteins in pancreatic alpha and beta cell lines. / Clark, Andy; Petersen, H V; Read, M L; Scott, V; Michelsen, B K; Docherty, K.

In: FEBS Letters, Vol. 329, No. 1-2, 23.08.1993, p. 139-43.

Research output: Contribution to journalArticlepeer-review

Harvard

Clark, A, Petersen, HV, Read, ML, Scott, V, Michelsen, BK & Docherty, K 1993, 'Human insulin gene enhancer-binding proteins in pancreatic alpha and beta cell lines', FEBS Letters, vol. 329, no. 1-2, pp. 139-43.

APA

Clark, A., Petersen, H. V., Read, M. L., Scott, V., Michelsen, B. K., & Docherty, K. (1993). Human insulin gene enhancer-binding proteins in pancreatic alpha and beta cell lines. FEBS Letters, 329(1-2), 139-43.

Vancouver

Clark A, Petersen HV, Read ML, Scott V, Michelsen BK, Docherty K. Human insulin gene enhancer-binding proteins in pancreatic alpha and beta cell lines. FEBS Letters. 1993 Aug 23;329(1-2):139-43.

Author

Clark, Andy ; Petersen, H V ; Read, M L ; Scott, V ; Michelsen, B K ; Docherty, K. / Human insulin gene enhancer-binding proteins in pancreatic alpha and beta cell lines. In: FEBS Letters. 1993 ; Vol. 329, No. 1-2. pp. 139-43.

Bibtex

@article{3f71a001ca6149b1ab583c6512b1abe4,
title = "Human insulin gene enhancer-binding proteins in pancreatic alpha and beta cell lines",
abstract = "Electrophoretic mobility shift assays were performed using oligonucleotides corresponding to known protein binding sites within the human insulin gene enhancer and nuclear extracts from mouse pancreatic alpha and beta cell lines. The results demonstrate that a previously described factor, IUF-1, binds to three sites at -82 (the CT1 box), -215 (the CT2 box), and -319 (the CT3 box) in the human insulin gene enhancer. IUF-1 was present only in beta but not in alpha cells, while all other DNA-binding proteins were present in both cell lines. IUF-1 may therefore be an important determinant of insulin gene beta cell-specific expression.",
keywords = "Animals, Base Sequence, Cell Line, Cell Nucleus, DNA, DNA-Binding Proteins, Enhancer Elements, Genetic, Humans, Insulin, Mice, Mice, Transgenic, Molecular Sequence Data, Oligonucleotide Probes, Pancreas",
author = "Andy Clark and Petersen, {H V} and Read, {M L} and V Scott and Michelsen, {B K} and K Docherty",
year = "1993",
month = aug,
day = "23",
language = "English",
volume = "329",
pages = "139--43",
journal = "FEBS Letters",
issn = "0014-5793",
publisher = "Elsevier",
number = "1-2",

}

RIS

TY - JOUR

T1 - Human insulin gene enhancer-binding proteins in pancreatic alpha and beta cell lines

AU - Clark, Andy

AU - Petersen, H V

AU - Read, M L

AU - Scott, V

AU - Michelsen, B K

AU - Docherty, K

PY - 1993/8/23

Y1 - 1993/8/23

N2 - Electrophoretic mobility shift assays were performed using oligonucleotides corresponding to known protein binding sites within the human insulin gene enhancer and nuclear extracts from mouse pancreatic alpha and beta cell lines. The results demonstrate that a previously described factor, IUF-1, binds to three sites at -82 (the CT1 box), -215 (the CT2 box), and -319 (the CT3 box) in the human insulin gene enhancer. IUF-1 was present only in beta but not in alpha cells, while all other DNA-binding proteins were present in both cell lines. IUF-1 may therefore be an important determinant of insulin gene beta cell-specific expression.

AB - Electrophoretic mobility shift assays were performed using oligonucleotides corresponding to known protein binding sites within the human insulin gene enhancer and nuclear extracts from mouse pancreatic alpha and beta cell lines. The results demonstrate that a previously described factor, IUF-1, binds to three sites at -82 (the CT1 box), -215 (the CT2 box), and -319 (the CT3 box) in the human insulin gene enhancer. IUF-1 was present only in beta but not in alpha cells, while all other DNA-binding proteins were present in both cell lines. IUF-1 may therefore be an important determinant of insulin gene beta cell-specific expression.

KW - Animals

KW - Base Sequence

KW - Cell Line

KW - Cell Nucleus

KW - DNA

KW - DNA-Binding Proteins

KW - Enhancer Elements, Genetic

KW - Humans

KW - Insulin

KW - Mice

KW - Mice, Transgenic

KW - Molecular Sequence Data

KW - Oligonucleotide Probes

KW - Pancreas

M3 - Article

C2 - 8354388

VL - 329

SP - 139

EP - 143

JO - FEBS Letters

JF - FEBS Letters

SN - 0014-5793

IS - 1-2

ER -