Human insulin gene enhancer-binding proteins in pancreatic alpha and beta cell lines

Research output: Contribution to journalArticle

Authors

  • H V Petersen
  • V Scott
  • B K Michelsen
  • K Docherty

Colleges, School and Institutes

Abstract

Electrophoretic mobility shift assays were performed using oligonucleotides corresponding to known protein binding sites within the human insulin gene enhancer and nuclear extracts from mouse pancreatic alpha and beta cell lines. The results demonstrate that a previously described factor, IUF-1, binds to three sites at -82 (the CT1 box), -215 (the CT2 box), and -319 (the CT3 box) in the human insulin gene enhancer. IUF-1 was present only in beta but not in alpha cells, while all other DNA-binding proteins were present in both cell lines. IUF-1 may therefore be an important determinant of insulin gene beta cell-specific expression.

Details

Original languageEnglish
Pages (from-to)139-43
Number of pages5
JournalFEBS Letters
Volume329
Issue number1-2
Publication statusPublished - 23 Aug 1993

Keywords

  • Animals, Base Sequence, Cell Line, Cell Nucleus, DNA, DNA-Binding Proteins, Enhancer Elements, Genetic, Humans, Insulin, Mice, Mice, Transgenic, Molecular Sequence Data, Oligonucleotide Probes, Pancreas