Histopathologic features of inflammatory bowel disease are associated with different CD4+ T cell subsets in colonic mucosal lamina propria

Research output: Contribution to journalArticlepeer-review

Standard

Histopathologic features of inflammatory bowel disease are associated with different CD4+ T cell subsets in colonic mucosal lamina propria. / Iacucci, Marietta; Gui, Xianyong; Li, Ji; Ueno, Aito; Qian, Jiaming; Ghosh, Subrata.

In: Journal of Crohn's & Colitis, 2018.

Research output: Contribution to journalArticlepeer-review

Harvard

APA

Vancouver

Author

Bibtex

@article{a9fba7190dc74b17b77eb57eb4c03b17,
title = "Histopathologic features of inflammatory bowel disease are associated with different CD4+ T cell subsets in colonic mucosal lamina propria",
abstract = "Background: Inflammatory bowel disease (IBD) results particularly from aberrance of CD4+ helper and regulatory T cells and is histopathologically chronic active enterocolitis with features reflecting both activity and chronicity of mucosal inflammation. The exact immunologic-histologic correlation is not understood.Methods: We studied the correlation between colonic mucosal CD4+ T cell subsets (Th1, Th2, Th17, Th22, and Treg) and mucosal histologic changes in ulcerative colitis (UC) and Crohn's disease (CD). CD4+ T cell subtyping and enumeration were achieved by flow cytometry. Histologic features were categorized and semi-quantitated using three validated histological scoring schemes (ECAP, RHI, and D'Haens). Correlations between prevalence (%) of CD4+ T cell subsets and histologic scores were analyzed.Results: Treg cells were correlated with ECAP category A (activity) as well as RHI scores. Treg was particularly increased in mucosa with severe neutrophilic infiltration in cryptal/surface epithelium and in lamina propria, and with basal plasmacytosis. Th17 cells were also increased in cases with extensive neutrophil infiltrate in lamina propria, whereas RORc+ cells were increased in cases with severe lymphoplasmacytic infiltration in lamina propria. In both UC and CD, the mucosa with marked crypt architectural alteration had increased IL-22+ and Th22 cells. UC with Paneth cell metaplasia had higher Th17 cells. CD with granuloma had increased IL-22+ and IL-22+IFN-γ+ cells.Conclusions: Treg appears to be associated with the overall severity of IBD histopathology, particularly with active inflammation. Th17 is also associated with activity. Whereas IL-22+ cells are associated with chronicity and granuloma formation in CD.",
author = "Marietta Iacucci and Xianyong Gui and Ji Li and Aito Ueno and Jiaming Qian and Subrata Ghosh",
year = "2018",
doi = "10.1093/ecco-jcc/jjy116",
language = "English",
journal = "Journal of Crohn's & Colitis",
issn = "1873-9946",
publisher = "Oxford University Press",

}

RIS

TY - JOUR

T1 - Histopathologic features of inflammatory bowel disease are associated with different CD4+ T cell subsets in colonic mucosal lamina propria

AU - Iacucci, Marietta

AU - Gui, Xianyong

AU - Li, Ji

AU - Ueno, Aito

AU - Qian, Jiaming

AU - Ghosh, Subrata

PY - 2018

Y1 - 2018

N2 - Background: Inflammatory bowel disease (IBD) results particularly from aberrance of CD4+ helper and regulatory T cells and is histopathologically chronic active enterocolitis with features reflecting both activity and chronicity of mucosal inflammation. The exact immunologic-histologic correlation is not understood.Methods: We studied the correlation between colonic mucosal CD4+ T cell subsets (Th1, Th2, Th17, Th22, and Treg) and mucosal histologic changes in ulcerative colitis (UC) and Crohn's disease (CD). CD4+ T cell subtyping and enumeration were achieved by flow cytometry. Histologic features were categorized and semi-quantitated using three validated histological scoring schemes (ECAP, RHI, and D'Haens). Correlations between prevalence (%) of CD4+ T cell subsets and histologic scores were analyzed.Results: Treg cells were correlated with ECAP category A (activity) as well as RHI scores. Treg was particularly increased in mucosa with severe neutrophilic infiltration in cryptal/surface epithelium and in lamina propria, and with basal plasmacytosis. Th17 cells were also increased in cases with extensive neutrophil infiltrate in lamina propria, whereas RORc+ cells were increased in cases with severe lymphoplasmacytic infiltration in lamina propria. In both UC and CD, the mucosa with marked crypt architectural alteration had increased IL-22+ and Th22 cells. UC with Paneth cell metaplasia had higher Th17 cells. CD with granuloma had increased IL-22+ and IL-22+IFN-γ+ cells.Conclusions: Treg appears to be associated with the overall severity of IBD histopathology, particularly with active inflammation. Th17 is also associated with activity. Whereas IL-22+ cells are associated with chronicity and granuloma formation in CD.

AB - Background: Inflammatory bowel disease (IBD) results particularly from aberrance of CD4+ helper and regulatory T cells and is histopathologically chronic active enterocolitis with features reflecting both activity and chronicity of mucosal inflammation. The exact immunologic-histologic correlation is not understood.Methods: We studied the correlation between colonic mucosal CD4+ T cell subsets (Th1, Th2, Th17, Th22, and Treg) and mucosal histologic changes in ulcerative colitis (UC) and Crohn's disease (CD). CD4+ T cell subtyping and enumeration were achieved by flow cytometry. Histologic features were categorized and semi-quantitated using three validated histological scoring schemes (ECAP, RHI, and D'Haens). Correlations between prevalence (%) of CD4+ T cell subsets and histologic scores were analyzed.Results: Treg cells were correlated with ECAP category A (activity) as well as RHI scores. Treg was particularly increased in mucosa with severe neutrophilic infiltration in cryptal/surface epithelium and in lamina propria, and with basal plasmacytosis. Th17 cells were also increased in cases with extensive neutrophil infiltrate in lamina propria, whereas RORc+ cells were increased in cases with severe lymphoplasmacytic infiltration in lamina propria. In both UC and CD, the mucosa with marked crypt architectural alteration had increased IL-22+ and Th22 cells. UC with Paneth cell metaplasia had higher Th17 cells. CD with granuloma had increased IL-22+ and IL-22+IFN-γ+ cells.Conclusions: Treg appears to be associated with the overall severity of IBD histopathology, particularly with active inflammation. Th17 is also associated with activity. Whereas IL-22+ cells are associated with chronicity and granuloma formation in CD.

U2 - 10.1093/ecco-jcc/jjy116

DO - 10.1093/ecco-jcc/jjy116

M3 - Article

C2 - 30137280

JO - Journal of Crohn's & Colitis

JF - Journal of Crohn's & Colitis

SN - 1873-9946

ER -