High-throughput whole-genome sequencing to dissect the epidemiology of Acinetobacter baumannii isolates from a hospital outbreak.
Research output: Contribution to journal › Article
Colleges, School and Institutes
Shared care of military and civilian patients has resulted in transmission of multidrug-resistant Acinetobacter baumannii (MDR-Aci) from military casualties to civilians. Current typing technologies have been useful in revealing relationships between isolates of A. baumannii but they are unable to resolve differences between closely related isolates from small-scale outbreaks, where chains of transmission are often unclear. In a recent hospital outbreak in Birmingham, six patients were colonised with MDR-Aci isolates indistinguishable using standard techniques. We used whole-genome sequencing to identify single nucleotide polymorphisms in these isolates, allowing us to discriminate between alternative epidemiological hypotheses in this setting.
|Number of pages||5|
|Journal||The Journal of hospital infection|
|Publication status||Published - 1 May 2010|
- Acinetobacter baumannii, Single nucleotide polymorphism, Epidemiology, Multidrug resistance, 454 pyrosequencing, Whole-genome sequencing