High-density protein loading on hierarchically porous layered double hydroxide composites with a rational mesostructure

Research output: Contribution to journalArticle

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High-density protein loading on hierarchically porous layered double hydroxide composites with a rational mesostructure. / Tokudome, Yasuaki; Fukui, Megu; Tarutani, Naoki; Nishimura, Sari; Prevot, Vanessa; Forano, Claude; Poologasundarampillai, Gowsihan; Lee, Peter D; Takahashi, Masahide.

In: Langmuir, Vol. 32, No. 35, 08.08.2016, p. 8826-8833.

Research output: Contribution to journalArticle

Harvard

Tokudome, Y, Fukui, M, Tarutani, N, Nishimura, S, Prevot, V, Forano, C, Poologasundarampillai, G, Lee, PD & Takahashi, M 2016, 'High-density protein loading on hierarchically porous layered double hydroxide composites with a rational mesostructure', Langmuir, vol. 32, no. 35, pp. 8826-8833. https://doi.org/10.1021/acs.langmuir.6b01925

APA

Tokudome, Y., Fukui, M., Tarutani, N., Nishimura, S., Prevot, V., Forano, C., Poologasundarampillai, G., Lee, P. D., & Takahashi, M. (2016). High-density protein loading on hierarchically porous layered double hydroxide composites with a rational mesostructure. Langmuir, 32(35), 8826-8833. https://doi.org/10.1021/acs.langmuir.6b01925

Vancouver

Author

Tokudome, Yasuaki ; Fukui, Megu ; Tarutani, Naoki ; Nishimura, Sari ; Prevot, Vanessa ; Forano, Claude ; Poologasundarampillai, Gowsihan ; Lee, Peter D ; Takahashi, Masahide. / High-density protein loading on hierarchically porous layered double hydroxide composites with a rational mesostructure. In: Langmuir. 2016 ; Vol. 32, No. 35. pp. 8826-8833.

Bibtex

@article{7785c9d7f92d434791decf2ccb29775d,
title = "High-density protein loading on hierarchically porous layered double hydroxide composites with a rational mesostructure",
abstract = "Hierarchically porous biocompatible Mg–Al–Cl-type layered double hydroxide (LDH) composites containing aluminum hydroxide (Alhy) have been prepared using a phase-separation process. The sol–gel synthesis allows for the hierarchical pores of the LDH–Alhy composites to be tuned, leading to a high specific solid surface area per unit volume available for high-molecular-weight protein adsorptions. A linear relationship between the effective surface area, SEFF, and loading capacity of a model protein, bovine serum albumin (BSA), is established following successful control of the structure of the LDH–Alhy composite. The threshold of the mean pore diameter, Dpm, above which BSA is effectively adsorbed on the surface of LDH–Alhy composites, is deduced as 20 nm. In particular, LDH–Alhy composite aerogels obtained via supercritical drying exhibit an extremely high capacity for protein loading (996 mg/g) as a result of a large mean mesopore diameter (>30 nm). The protein loading on LDH–Alhy is >14 times that of a reference LDH material (70 mg/g) prepared via a standard procedure. Importantly, BSA molecules pre-adsorbed on porous composites were successfully released on soaking in ionic solutions (HPO42– and Cl– aqueous). The superior capability of the biocompatible LDH materials for loading, encapsulation, and releasing large quantities of proteins was clearly demonstrated.",
author = "Yasuaki Tokudome and Megu Fukui and Naoki Tarutani and Sari Nishimura and Vanessa Prevot and Claude Forano and Gowsihan Poologasundarampillai and Lee, {Peter D} and Masahide Takahashi",
year = "2016",
month = aug,
day = "8",
doi = "10.1021/acs.langmuir.6b01925",
language = "English",
volume = "32",
pages = "8826--8833",
journal = "Langmuir",
issn = "0743-7463",
publisher = "American Chemical Society",
number = "35",

}

RIS

TY - JOUR

T1 - High-density protein loading on hierarchically porous layered double hydroxide composites with a rational mesostructure

AU - Tokudome, Yasuaki

AU - Fukui, Megu

AU - Tarutani, Naoki

AU - Nishimura, Sari

AU - Prevot, Vanessa

AU - Forano, Claude

AU - Poologasundarampillai, Gowsihan

AU - Lee, Peter D

AU - Takahashi, Masahide

PY - 2016/8/8

Y1 - 2016/8/8

N2 - Hierarchically porous biocompatible Mg–Al–Cl-type layered double hydroxide (LDH) composites containing aluminum hydroxide (Alhy) have been prepared using a phase-separation process. The sol–gel synthesis allows for the hierarchical pores of the LDH–Alhy composites to be tuned, leading to a high specific solid surface area per unit volume available for high-molecular-weight protein adsorptions. A linear relationship between the effective surface area, SEFF, and loading capacity of a model protein, bovine serum albumin (BSA), is established following successful control of the structure of the LDH–Alhy composite. The threshold of the mean pore diameter, Dpm, above which BSA is effectively adsorbed on the surface of LDH–Alhy composites, is deduced as 20 nm. In particular, LDH–Alhy composite aerogels obtained via supercritical drying exhibit an extremely high capacity for protein loading (996 mg/g) as a result of a large mean mesopore diameter (>30 nm). The protein loading on LDH–Alhy is >14 times that of a reference LDH material (70 mg/g) prepared via a standard procedure. Importantly, BSA molecules pre-adsorbed on porous composites were successfully released on soaking in ionic solutions (HPO42– and Cl– aqueous). The superior capability of the biocompatible LDH materials for loading, encapsulation, and releasing large quantities of proteins was clearly demonstrated.

AB - Hierarchically porous biocompatible Mg–Al–Cl-type layered double hydroxide (LDH) composites containing aluminum hydroxide (Alhy) have been prepared using a phase-separation process. The sol–gel synthesis allows for the hierarchical pores of the LDH–Alhy composites to be tuned, leading to a high specific solid surface area per unit volume available for high-molecular-weight protein adsorptions. A linear relationship between the effective surface area, SEFF, and loading capacity of a model protein, bovine serum albumin (BSA), is established following successful control of the structure of the LDH–Alhy composite. The threshold of the mean pore diameter, Dpm, above which BSA is effectively adsorbed on the surface of LDH–Alhy composites, is deduced as 20 nm. In particular, LDH–Alhy composite aerogels obtained via supercritical drying exhibit an extremely high capacity for protein loading (996 mg/g) as a result of a large mean mesopore diameter (>30 nm). The protein loading on LDH–Alhy is >14 times that of a reference LDH material (70 mg/g) prepared via a standard procedure. Importantly, BSA molecules pre-adsorbed on porous composites were successfully released on soaking in ionic solutions (HPO42– and Cl– aqueous). The superior capability of the biocompatible LDH materials for loading, encapsulation, and releasing large quantities of proteins was clearly demonstrated.

U2 - 10.1021/acs.langmuir.6b01925

DO - 10.1021/acs.langmuir.6b01925

M3 - Article

VL - 32

SP - 8826

EP - 8833

JO - Langmuir

JF - Langmuir

SN - 0743-7463

IS - 35

ER -