Abstract
How the cellular immune response copes with diverse antigenic competition is poorly understood. Responses of virus-specific cytotoxic T lymphocytes (CTL) were examined longitudinally in an individual coinfected with human immunodeficiency virus type 1 (HIV-1), Epstein-Barr virus (EBV), and cytomegalovirus (CMV). CTL responses to all 3 viruses were quantified by limiting dilution analysis and staining with HLA-A*0201 tetrameric complexes folded with HIV-1, EBV, and CMV peptides. A predominance of CMV-pp65-specific CTL was found, with a much lower frequency of CTL to HIV-1 Gag and Pol and to EBV-BMLF1 and LMP2. The high frequency of CMV-specific CTL, compared with HIV-1- and EBV-specific CTL, was confirmed in an additional 16 HLA-A*0201-positive virus-coinfected subjects. Therefore, the human immune system can mount CTL responses to multiple viral antigens simultaneously, albeit with different strengths.
Original language | English |
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Pages (from-to) | 165-75 |
Number of pages | 11 |
Journal | The Journal of Infectious Diseases |
Volume | 181 |
Issue number | 1 |
DOIs | |
Publication status | Published - Jan 2000 |
Keywords
- Adult
- Cross-Sectional Studies
- Cytomegalovirus
- Cytomegalovirus Infections
- Cytotoxicity, Immunologic
- Epstein-Barr Virus Infections
- HIV Infections
- HIV-1
- HLA-A Antigens
- Herpesviridae Infections
- Herpesvirus 4, Human
- Humans
- Male
- T-Lymphocytes