High frequency of cytomegalovirus-specific cytotoxic T-effector cells in HLA-A*0201-positive subjects during multiple viral coinfections

X Jin; M A Demoitie; S M Donahoe; G S Ogg; S Bonhoeffer; W M Kakimoto; G Gillespie; P A Moss; W Dyer; M G Kurilla; S R Riddell; J Downie; J S Sullivan; A J McMichael; C Workman; D F Nixon; Sindhu Patil

doi:10.1086/315201

High frequency of cytomegalovirus-specific cytotoxic T-effector cells in HLA-A*0201-positive subjects during multiple viral coinfections

X Jin, M A Demoitie, S M Donahoe, G S Ogg, S Bonhoeffer, W M Kakimoto, G Gillespie, P A Moss, W Dyer, M G Kurilla, S R Riddell, J Downie, J S Sullivan, A J McMichael, C Workman, D F Nixon, Sindhu Patil

Cancer and Genomic Sciences

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Abstract

How the cellular immune response copes with diverse antigenic competition is poorly understood. Responses of virus-specific cytotoxic T lymphocytes (CTL) were examined longitudinally in an individual coinfected with human immunodeficiency virus type 1 (HIV-1), Epstein-Barr virus (EBV), and cytomegalovirus (CMV). CTL responses to all 3 viruses were quantified by limiting dilution analysis and staining with HLA-A*0201 tetrameric complexes folded with HIV-1, EBV, and CMV peptides. A predominance of CMV-pp65-specific CTL was found, with a much lower frequency of CTL to HIV-1 Gag and Pol and to EBV-BMLF1 and LMP2. The high frequency of CMV-specific CTL, compared with HIV-1- and EBV-specific CTL, was confirmed in an additional 16 HLA-A*0201-positive virus-coinfected subjects. Therefore, the human immune system can mount CTL responses to multiple viral antigens simultaneously, albeit with different strengths.

Original language	English
Pages (from-to)	165-75
Number of pages	11
Journal	The Journal of Infectious Diseases
Volume	181
Issue number	1
DOIs	https://doi.org/10.1086/315201
Publication status	Published - Jan 2000

Keywords

Adult
Cross-Sectional Studies
Cytomegalovirus
Cytomegalovirus Infections
Cytotoxicity, Immunologic
Epstein-Barr Virus Infections
HIV Infections
HIV-1
HLA-A Antigens
Herpesviridae Infections
Herpesvirus 4, Human
Humans
Male
T-Lymphocytes

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1086/315201

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Jin, X., Demoitie, M. A., Donahoe, S. M., Ogg, G. S., Bonhoeffer, S., Kakimoto, W. M., Gillespie, G., Moss, P. A., Dyer, W., Kurilla, M. G., Riddell, S. R., Downie, J., Sullivan, J. S., McMichael, A. J., Workman, C., Nixon, D. F., & Patil, S. (2000). High frequency of cytomegalovirus-specific cytotoxic T-effector cells in HLA-A*0201-positive subjects during multiple viral coinfections. The Journal of Infectious Diseases, 181(1), 165-75. https://doi.org/10.1086/315201

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title = "High frequency of cytomegalovirus-specific cytotoxic T-effector cells in HLA-A*0201-positive subjects during multiple viral coinfections",

abstract = "How the cellular immune response copes with diverse antigenic competition is poorly understood. Responses of virus-specific cytotoxic T lymphocytes (CTL) were examined longitudinally in an individual coinfected with human immunodeficiency virus type 1 (HIV-1), Epstein-Barr virus (EBV), and cytomegalovirus (CMV). CTL responses to all 3 viruses were quantified by limiting dilution analysis and staining with HLA-A*0201 tetrameric complexes folded with HIV-1, EBV, and CMV peptides. A predominance of CMV-pp65-specific CTL was found, with a much lower frequency of CTL to HIV-1 Gag and Pol and to EBV-BMLF1 and LMP2. The high frequency of CMV-specific CTL, compared with HIV-1- and EBV-specific CTL, was confirmed in an additional 16 HLA-A*0201-positive virus-coinfected subjects. Therefore, the human immune system can mount CTL responses to multiple viral antigens simultaneously, albeit with different strengths.",

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year = "2000",

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doi = "10.1086/315201",

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Jin, X, Demoitie, MA, Donahoe, SM, Ogg, GS, Bonhoeffer, S, Kakimoto, WM, Gillespie, G, Moss, PA, Dyer, W, Kurilla, MG, Riddell, SR, Downie, J, Sullivan, JS, McMichael, AJ, Workman, C, Nixon, DF & Patil, S 2000, 'High frequency of cytomegalovirus-specific cytotoxic T-effector cells in HLA-A*0201-positive subjects during multiple viral coinfections', The Journal of Infectious Diseases, vol. 181, no. 1, pp. 165-75. https://doi.org/10.1086/315201

TY - JOUR

T1 - High frequency of cytomegalovirus-specific cytotoxic T-effector cells in HLA-A*0201-positive subjects during multiple viral coinfections

AU - Jin, X

AU - Demoitie, M A

AU - Donahoe, S M

AU - Ogg, G S

AU - Bonhoeffer, S

AU - Kakimoto, W M

AU - Gillespie, G

AU - Moss, P A

AU - Dyer, W

AU - Kurilla, M G

AU - Riddell, S R

AU - Downie, J

AU - Sullivan, J S

AU - McMichael, A J

AU - Workman, C

AU - Nixon, D F

AU - Patil, Sindhu

PY - 2000/1

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N2 - How the cellular immune response copes with diverse antigenic competition is poorly understood. Responses of virus-specific cytotoxic T lymphocytes (CTL) were examined longitudinally in an individual coinfected with human immunodeficiency virus type 1 (HIV-1), Epstein-Barr virus (EBV), and cytomegalovirus (CMV). CTL responses to all 3 viruses were quantified by limiting dilution analysis and staining with HLA-A*0201 tetrameric complexes folded with HIV-1, EBV, and CMV peptides. A predominance of CMV-pp65-specific CTL was found, with a much lower frequency of CTL to HIV-1 Gag and Pol and to EBV-BMLF1 and LMP2. The high frequency of CMV-specific CTL, compared with HIV-1- and EBV-specific CTL, was confirmed in an additional 16 HLA-A*0201-positive virus-coinfected subjects. Therefore, the human immune system can mount CTL responses to multiple viral antigens simultaneously, albeit with different strengths.

AB - How the cellular immune response copes with diverse antigenic competition is poorly understood. Responses of virus-specific cytotoxic T lymphocytes (CTL) were examined longitudinally in an individual coinfected with human immunodeficiency virus type 1 (HIV-1), Epstein-Barr virus (EBV), and cytomegalovirus (CMV). CTL responses to all 3 viruses were quantified by limiting dilution analysis and staining with HLA-A*0201 tetrameric complexes folded with HIV-1, EBV, and CMV peptides. A predominance of CMV-pp65-specific CTL was found, with a much lower frequency of CTL to HIV-1 Gag and Pol and to EBV-BMLF1 and LMP2. The high frequency of CMV-specific CTL, compared with HIV-1- and EBV-specific CTL, was confirmed in an additional 16 HLA-A*0201-positive virus-coinfected subjects. Therefore, the human immune system can mount CTL responses to multiple viral antigens simultaneously, albeit with different strengths.

KW - Adult

KW - Cross-Sectional Studies

KW - Cytomegalovirus

KW - Cytomegalovirus Infections

KW - Cytotoxicity, Immunologic

KW - Epstein-Barr Virus Infections

KW - HIV Infections

KW - HIV-1

KW - HLA-A Antigens

KW - Herpesviridae Infections

KW - Herpesvirus 4, Human

KW - Humans

KW - Male

KW - T-Lymphocytes

U2 - 10.1086/315201

DO - 10.1086/315201

M3 - Article

C2 - 10608763

SN - 0022-1899

VL - 181

SP - 165

EP - 175

JO - The Journal of Infectious Diseases

JF - The Journal of Infectious Diseases

IS - 1

ER -

High frequency of cytomegalovirus-specific cytotoxic T-effector cells in HLA-A*0201-positive subjects during multiple viral coinfections

Abstract

Keywords

UN SDGs

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